PMID: 9525576Apr 3, 1998Paper

Glucagon treatment interferes with an early step of duck hepatitis B virus infection

Journal of Virology
M HildH Schaller

Abstract

The effect of glucagon on the establishment of hepadnavirus infection was studied in vitro with the duck hepatitis B virus (DHBV) model. The presence of the peptide hormone throughout infection or starting up to 8 h after virus uptake resulted in a dose-dependent reduction in the levels of intra- and extracellular viral gene products and of secreted virions. Treatment with forskolin or dibutyryl-cyclic AMP, two drugs that also stimulate the cyclic AMP (cAMP) signal transduction pathway, resulted in comparable inhibition, suggesting that the inhibitor effect is related to changes in the activity of protein kinase A. In persistently infected hepatocytes, only a slight, but continuous, decrease in viral replication was observed upon prolonged drug treatment. Time course analysis, including detection of DHBV covalently closed circular (ccc) DNA templates, revealed that glucagon acts late during the establishment of infection, at a time when the virus is already internalized, but before detectable ccc DNA accumulation in the nucleus. These data suggest that nuclear import (and reimport) of DHBV DNA genomes from cytosolic capsids is subject to cAMP-mediated regulation by cellular factors responding to changes in the state of the host...Continue Reading

References

May 1, 1992·Journal of Virology·R J Rigg, H Schaller
Oct 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P R GalleH Schaller
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Citations

Jul 11, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bo Kyung KimYun Gyu Park
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Stanca M CiupeAlan S Perelson
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·C Seeger, W S Mason
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·U ProtzerH Schaller

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