PMID: 9187537Feb 1, 1997Paper

Glucocorticoid effects on the skeletal muscle differentiation program: analysis of clonal proliferation, morphological differentiation and the expression of muscle-specific and regulatory genes

Endocrine Research
M M Montano, R W Lim

Abstract

We examined the effect of glucocorticoids on the proliferation and differentiation of skeletal muscle cells using the C2C12 cell line. We found that treatment with glucocorticoids enhanced muscle cell differentiation but had only minor effects on the clonal growth rate of C2C12 cells. The stimulatory effect of glucocorticoids on myogenic differentiation was reflected in the increased expression of muscle-specific genes, creatine kinase (CK) and acetylcholine receptor gamma subunit (AChR). Dexamethasone had no effect on CK and AChR mRNA stability and enhanced transcription from a CAT reporter genes containing the 3.3kb 5' flanking region of the murine CK gene (-3300MCK-CAT). Since dexamethasone did not affect the expression levels of the myogenic regulatory genes such as myoD and myogenin, the enhancement of muscle-specific transcription might reflect an increase in the functional activity of the regulatory proteins. Other possible mechanisms involved in the differentiation-enhancing effect of glucocorticoids are discussed.

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Citations

Sep 1, 2012·Journal of Applied Physiology·N A PanstersA M Schols
Dec 3, 2009·Circulation Journal : Official Journal of the Japanese Circulation Society·Masanori MizunoHitoshi Yamamoto
Jul 27, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C H WashabaughM Ontell
Dec 22, 1999·Neurobiology of Disease·M M RichR L Barchi

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