PMID: 11325615Apr 28, 2001Paper

Glucocorticoid enhances the response of type II cells from newborn rats to surfactant secretagogues

Biochimica Et Biophysica Acta
Y Isohama, S A Rooney

Abstract

There is a developmental increase in agonist-induced surfactant secretion in type II cells. The response to the P2Y(2) agonist UTP is negligible in early newborn cells but increases with age. The response to terbutaline, N-ethylcarboxyamidoadenosine (NECA), and ATP also increases with age. As glucocorticoids are known to accelerate several aspects of lung maturation we examined the effect of dexamethasone (Dex) on the response of 1-day-old rat type II cells to surfactant secretagogues. Freshly isolated cells were cultured +/-10(-6) M Dex for 18--20 h after which phosphatidylcholine secretion was measured. Dex slightly decreased the basal secretion rate. However, it significantly increased the response to terbutaline, NECA, ATP and UTP. This effect was dependent on Dex concentration (EC(50)=2-6 x 10(-9) M) and blocked by the glucocorticoid receptor antagonist RU-486. It is unlikely to be due to increased receptor content as Dex had no effect on adenylate cyclase, phospholipase C or phospholipase D activation and the response to cAMP, forskolin and phorbol ester, secretagogues acting downstream from receptors, was also increased by Dex. These data show that Dex acts directly on the type II cell to enhance the response to surfacta...Continue Reading

References

May 1, 1978·Endocrinology·P L BallardW H Douglas
Jan 1, 1991·Annual Review of Physiology·J R Wright, L G Dobbs
Feb 3, 1986·Life Sciences·M FujisawaF Takaku
Dec 16, 1980·Biochemical and Biophysical Research Communications·J A WhitsettH Needelman

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Citations

Jun 7, 2011·Respiratory Physiology & Neurobiology·Sandra OrgeigChristopher B Daniels
Aug 3, 2004·Journal of Pharmacological Sciences·Tsutomu SakumaHirohisa Toga
Oct 12, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Lucy C SullivanChristopher B Daniels
Jun 20, 2020·Cell and Tissue Research·Kazuhiro OsanaiKeiji Takahashi
Dec 26, 2001·Biochimica Et Biophysica Acta·S Beneke, S A Rooney

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