PMID: 9831479Sep 1, 1993Paper

Glucocorticoid receptor binding to rat liver nuclei occurs without nuclear transport

The Journal of Steroid Biochemistry and Molecular Biology
Y MiyashitaS S Simons


The binding of cell-free activated glucocorticoid receptor-steroid complexes from HTC cells to various preparations of HTC and rat liver nuclei has been examined under conditions that did or did not support the nuclear translocation of macromolecules via nuclear pores. To the best of our knowledge, this is the first such study with functionally active isolated nuclei. Conventionally prepared HTC nuclei were found to be porous, as determined from their inability to exclude the fluorescent macromolecule phycoerythrin (PE) at 4 degrees C. Thus the nuclear binding of activated complexes to these nuclei can not involve nuclear translocation. Further studies, using established conditions with sealed nuclei prepared from rat liver, revealed that nuclear translocation of PE containing a covalently linked, authentic nuclear translocation sequence could be obtained at 22 degrees C, but not at 4 degrees C. However, under the same conditions, activated glucocorticoid complexes displayed equal levels of nuclear binding at both temperatures. We therefore conclude that the current translocation conditions with intact rat liver nuclei are not sufficient to reproduce the nuclear transport of glucocorticoid complexes observed in intact cells. Th...Continue Reading


Feb 13, 1978·Biochimica Et Biophysica Acta·M Atger, E Milgrom
Aug 7, 1992·Cell·T Hunter, M Karin
Jul 1, 1991·Archives of Biochemistry and Biophysics·B WolffJ A Hanover
Dec 1, 1991·Trends in Biochemical Sciences·C Dingwall, R A Laskey
Nov 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·G S TakimotoK B Horwitz
Jan 15, 1992·Biochemical and Biophysical Research Communications·F HamyJ P Henichart
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·E C LaCasse, Y A Lefebvre
Feb 8, 1991·Cell·P A Silver
Mar 7, 1991·Biochimica Et Biophysica Acta·J Garcia-BustosM N Hall
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M S Halleck, M Rechsteiner
Jan 1, 1986·Methods in Enzymology·M Z Atassi
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J O BendittN Riedel
Dec 1, 1986·The Journal of Cell Biology·D D NewmeyerD J Forbes
Oct 1, 1988·Experimental Cell Research·B WolffJ A Hanover
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N Imamoto-SonobeT Uchida
Jan 1, 1986·Journal of Steroid Biochemistry·S S Simons, P A Miller
Aug 1, 1985·Journal of Steroid Biochemistry·V K Moudgil, V M Caradonna
Dec 10, 1973·Biochemical and Biophysical Research Communications·T Blumenthal, T A Landers
Dec 1, 1982·Journal of Steroid Biochemistry·V K Moudgil, V M Caradonna

❮ Previous
Next ❯


Jul 1, 1994·Molecular Membrane Biology·J O BustamanteJ A Hanover
Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J O Bustamante, W A Varanda

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.