PMID: 9174122May 1, 1997Paper

Glucocorticoid receptor function possibly modulates cell-cell interactions in osteoblastic metastases on rat skeleton

Clinical & Experimental Metastasis
C Reyes-Moreno, M Koutsilieris

Abstract

We analysed the glucocorticoid receptor (GR) function and its ability to modulate cell-cell interactions between the PA-III rat prostate cancer and UMR 106 osteoblast-like rat osteosarcoma cells as an in vitro model for studying GR function in PA-III cell-induced tumor and blastic reaction in rat bone. Intact GR was detected by ligand binding assays, DNA band-shift, and GR trans-activation analysis of PA-III and UMR 106 cells transiently transfected with the mouse mammary tumor virus thymidine kinase-chloramphenicol acetyltransferase reporter gene. Dexamethasone and transforming growth factor beta 1 (TGFbeta1) inhibited the growth of PA-III and UMR 106 cells. Dexamethasone's inhibition of PA-III and UMR 106 cells was reversed by anti-TGFbeta1 antibody and exogenous insulin-like growth factor I (IGF-I). Exogenous IGF-I, urokinase-type plasminogen activator (uPA), UMR 106 conditioned media (CM) and PA-III CM stimulated the proliferation of PA-III and UMR 106 cells. The mitogenic activity exerted by uPA and PA-III CM in UMR 106 cells was completely neutralized by anti-IGF-I specific antibody. In addition, dexamethasone up-regulated TGFbeta1 mRNA and down-regulated uPA mRNA expression in PA-III cells without affecting TGFbeta1 and ...Continue Reading

Citations

Feb 7, 2002·Expert Opinion on Investigational Drugs·Michael KoutsilierisDimitrios Karamanolakis
Feb 5, 2009·Expert Opinion on Pharmacotherapy·Iraklis C MitsogiannisCharalambos Deliveliotis
Jan 5, 2002·Expert Opinion on Investigational Drugs·C S Mitsiades, M Koutsilieris
Mar 3, 2009·Pathology Oncology Research : POR·Sándor Békási, Attila Zalatnai
Mar 12, 2011·International Urology and Nephrology·Haruki KumeYukio Homma
Aug 25, 2005·Experimental Biology and Medicine·Morris Pollard, Mark A Suckow
Aug 15, 2009·Expert Opinion on Investigational Drugs·Pavlos MsaouelMichael Koutsilieris
Sep 13, 2013·Expert Opinion on Investigational Drugs·Pavlos MsaouelMichael Koutsilieris
Jul 9, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael KoutsilierisAthanassios Tsintavis

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