Glucocorticoid receptor-mediated regulation of MMP9 gene expression in human ovarian surface epithelial cells.

Fertility and Sterility
Michael T RaeStephen G Hillier

Abstract

To obtain proof-of-concept that locally produced anti-inflammatory steroids suppress ovulation-associated extracellular matrix proteases in human ovarian surface epithelial (OSE) cells. Primary OSE cell cultures treated with interleukin-1alpha (IL-1alpha) (500 pg/mL) as proxy for inflammation, with/without anti-inflammatory steroid (cortisol or progesterone [P], 0.01-1.0 microM). Academic medical center. Sixteen premenopausal women (29-46 years) undergoing surgery for nonmalignant gynecological conditions. Semiquantitative extracellular matrix protease gene expression profiling with verification by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and gelatinase zymography. Treatment with IL-1alpha stimulated messenger RNA (mRNA) expression of several ovulation-associated matrix metalloproteinase genes by OSE cell cultures, including gelatinase B (MMP9) but not gelatinase A (MMP2). The IL-1alpha-stimulated MMP9 mRNA production was suppressed by cortisol but not P. Cortisol but not P also dose-dependently suppressed IL-1alpha-stimulated MMP9 gelatinase activity and this effect was blocked by the glucocorticoid receptor antagonist RU486. In human OSE cells, stimulation of MMP9 gene expression and pr...Continue Reading

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Citations

Nov 17, 2015·Endocrinology and Metabolism Clinics of North America·Oksana LekarevMaria G Vogiatzi
Mar 31, 2009·Journal of Neuroimmunology·Lea Glass-MarmorAriel Miller
Dec 21, 2013·Fertility and Sterility·David E ReichmanZev Rosenwaks
Mar 29, 2014·Experimental Eye Research·Gangaraju RajashekharSangly P Srinivas
Nov 21, 2014·Biology of Reproduction·Jeffrey T ThornePhyllis C Leppert
Nov 30, 2018·Endocrine Reviews·Diane M DuffyThomas E Curry
Jan 11, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·A N AmwegH H Ortega
Dec 9, 2020·Molecular Aspects of Medicine·Camilla H K Hughes, Bruce D Murphy

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