Glucocorticoid receptors in the pig intestinal tract and muscle tissue

Zentralblatt für Veterinärmedizin. Reihe A
R ClausM Dehnhard

Abstract

Due to the known differences of protein turnover in skeletal muscle (slow turnover) and intestinal tract (rapid turnover), these tissues were analysed for the free glucocorticoid receptor (GR) concentrations. Cytosol was prepared from different parts of pig intestinal tract and two different muscles (longissimus thoracis: ML; trapezius: MT) under either energy restriction (5.8 MJ ME; 4 kg/d) or sufficient supply (14.0 MJ ME; 4 kg/d) for 4 days before sacrification. Care was taken that all samples were obtained within 15 min post-mortem and that premortem treatment did not elevate circulating cortisol. A Scatchard plot was performed both for muscle and gut tissue and the KD (0.4 +/- 0.04 nM; 0.7 +/- 0.02 nM) as well as Bmax (0.2 +/- 0.01 nM; 1.2 +/- 0.02 nM) pointed to specific binding. GR were generally high in intestinal tract irrespective of the anatomical site, but were significantly (P < or = 0.001) higher under energy restriction compared to adequate feeding (103.2 vs. 76.5 fmol/mg protein). GR was much lower in muscle tissues and tended to be slightly higher in MT (with high proportion of red fibres) compared to ML (white fibres) (12.4 vs. 9.1 fmol/mg protein). GR in muscles was not significantly influenced by energy. The...Continue Reading

References

Sep 1, 1975·Endocrinology·L Pressley, J W Funder
May 1, 1979·British Medical Bulletin·G C Liggins
Dec 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·H SauerweinH H Meyer
Sep 1, 1989·The Journal of Surgical Research·T B Morrissey, E A Woltering
Jun 24, 1985·Life Sciences·F Svec
Sep 1, 1987·Muscle & Nerve·J R Florini
Nov 1, 1985·Journal of Pharmacological Methods·G A McPherson
Jun 1, 1985·Australian and New Zealand Journal of Medicine·E A AbdiT A McPherson
Jul 1, 1981·Journal of Animal Science·M SnochowskiL E Edqvist
Oct 13, 1995·Biochemical and Biophysical Research Communications·T SakamotoF Hirata
Mar 1, 1994·Canadian Journal of Physiology and Pharmacology·R K Buddington
Mar 1, 1994·The American Journal of Physiology·G SchulmanC P Bastl
Feb 1, 1993·The Proceedings of the Nutrition Society·P J ReedsM L Fiorotto

❮ Previous
Next ❯

Citations

Jan 14, 2009·Journal of Animal Physiology and Animal Nutrition·A BauerR Claus
Jun 25, 2004·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·S O LopesR Mosenthin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.