Glucocorticoid sensitivity and inflammatory status of peripheral blood mononuclear cells in patients with coronary artery disease

Annals of Medicine
Simon JönssonLena Jonasson

Abstract

Mechanisms behind sustained inflammation in patients with coronary artery disease (CAD) are not clarified but hypothalamus-pituitary-adrenal (HPA) axis dysfunction may have a role. Here, we investigated whether inflammatory status of peripheral blood mononuclear cells (PBMCs) was associated with altered glucocorticoid sensitivity in CAD patients. In 55 CAD patients and 30 controls, mRNA levels of GR-α, GR-β, NF-κB, IκBα, MMP-9 and TIMP-1 were measured in PBMCs. Suppressive effects of dexamethasone on GR-α, GR-β, NF-κB, IκBα, MMP-9 and TIMP-1 mRNA levels were assessed in PBMCs ex vivo. Salivary cortisol was repeatedly measured over 3 days. GR-α mRNA levels were higher in CAD patients than in controls, 0.50 (0.38-0.59) versus 0.26 (0.18-0.37), p < .001, while GR-β mRNA levels were equally low in both groups. GR-α mRNA expression was associated with inflammatory gene expression and, also, with flatter diurnal cortisol rhythm. In both patients and controls, dexamethasone suppressed gene expression of NF-κB, IκBα, MMP-9 and TIMP-1 (p < .001). Dexamethasone also reduced GR-α mRNA levels (p < .001), while LPS increased it (p < .001). PBMCs from CAD patients displayed an inflammatory gene expression profile. This was not explained by r...Continue Reading

References

Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K BrownU Siebenlist
May 6, 1999·American Journal of Respiratory and Critical Care Medicine·Q A HamidD Y Leung
Mar 29, 2000·Brain : a Journal of Neurology·K A HarknessM N Woodroofe
May 25, 2002·European Journal of Clinical Investigation·P FantidisC Macaya
Aug 31, 2002·Journal of Gastroenterology and Hepatology·Takashiro HoriKitaro Oka
Sep 5, 2002·Biochemical and Biophysical Research Communications·Fumika OriiYutaka Kohgo
Jun 9, 2004·American Journal of Respiratory and Critical Care Medicine·Alfons TorregoCésar Picado
Oct 28, 2004·BMC Molecular Biology·Murilo R MeloCarlos A Longui
Feb 26, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Menno P J de WintherMarten H Hofker
Jan 3, 2006·American Journal of Respiratory and Critical Care Medicine·Elena GolevaDonald Y M Leung
Jul 21, 2006·Annals of the New York Academy of Sciences·Laura J Lewis-Tuffin, John A Cidlowski
Jan 16, 2007·BMC Immunology·Omar SharifNeil D Perkins
Jan 16, 2008·Archives of Internal Medicine·Erica L T van den AkkerSteven W J Lamberts
Jun 17, 2008·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·T S SilvaC E Kater
Jul 29, 2008·Journal of Psychosomatic Research·Mimi R BhattacharyyaAndrew Steptoe
Sep 6, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Andrew C Newby
Nov 4, 2008·Annals of Medicine·Johnny Nijm, Lena Jonasson
Feb 26, 2009·British Journal of Pharmacology·Patrick W F HadokeBrian R Walker
Feb 25, 2011·The Journal of Clinical Endocrinology and Metabolism·Meena KumariMika Kivimaki
May 20, 2011·Nature·Peter LibbyGöran K Hansson
Jul 25, 2012·Annals of the New York Academy of Sciences·Marni N Silverman, Esther M Sternberg
Oct 2, 2013·Nature Reviews. Endocrinology·Rogier A QuaxRichard A Feelders
Aug 20, 2014·Psychoneuroendocrinology·Suzanne C SegerstromSandra E Sephton
Mar 27, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Julie E GoodwinWilliam C Sessa
Mar 5, 2016·Journal of the American College of Cardiology·Christiane WallerHarald Gündel
Aug 29, 2017·The New England Journal of Medicine·Paul M RidkerUNKNOWN CANTOS Trial Group

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