PMID: 7537465Apr 1, 1995Paper

Glucocorticoid stimulation of fatty-acid synthase gene transcription in fetal lung: antagonism by retinoic acid

The American Journal of Physiology
Z X XuS A Rooney

Abstract

Glucocorticoid hormones are known to stimulate the rate of fatty acid biosynthesis and to increase the activity and mRNA level of fatty-acid synthase (FAS) in late gestation fetal lung. We have now examined the effect of dexamethasone on FAS transcription in fetal rat lung. Explants of 19-day fetal rat lung cultured for 48 h in serum-free medium were exposed to dexamethasone (10(-7) M) for various time periods. Nuclei were isolated, and the rate of [32P]UTP incorporation into FAS and gamma-actin RNA transcripts was measured by transcription-elongation assay. Dexamethasone increased FAS transcription but had no effect on that of actin. The maximum effect of the hormone, approximately threefold increase, was observed 1-2 h after addition of the hormone but was still apparent up to 48 h. FAS transcription but not that of actin was inhibited by cycloheximide and puromycin in both control and dexamethasone-treated cultures. However, the stimulatory effect of the hormone was not significantly reduced by the inhibitors. Retinoic acid antagonized the stimulatory effects of dexamethasone on FAS activity, mRNA content as measured by Northern analysis, mass as measured by Western blotting, and rate of transcription. The effect of retinoic...Continue Reading

Citations

Jun 1, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ross Ridsdale, Martin Post
Jun 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert J HomerJack A Elias
Dec 24, 2002·American Journal of Respiratory Cell and Molecular Biology·Feige KaplanNeil B Sweezey
Nov 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·L I Gobran, S A Rooney
Dec 26, 2001·Biochimica Et Biophysica Acta·S Beneke, S A Rooney
Jul 6, 2021·World Journal of Clinical Cases·Fu-Chen YangGuo-Xun Chen

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