Glucocorticoid-transactivated TSC22D3 attenuates hypoxia- and diabetes-induced Müller glial galectin-1 expression via HIF-1α destabilization.

Journal of Cellular and Molecular Medicine
Atsuhiro KandaSusumu Ishida

Abstract

Galectin-1/LGALS1, a newly recognized angiogenic factor, contributes to the pathogenesis of diabetic retinopathy (DR). Recently, we demonstrated that glucocorticoids suppressed an interleukin-1β-driven inflammatory pathway for galectin-1 expression in vitro and in vivo. Here, we show glucocorticoid-mediated inhibitory mechanism against hypoxia-inducible factor (HIF)-1α-involved galectin-1 expression in human Müller glial cells and the retina of diabetic mice. Hypoxia-induced increases in galectin-1/LGALS1 expression and promoter activity were attenuated by dexamethasone and triamcinolone acetonide in vitro. Glucocorticoid application to hypoxia-stimulated cells decreased HIF-1α protein, but not mRNA, together with its DNA-binding activity, while transactivating TSC22 domain family member (TSC22D)3 mRNA and protein expression. Co-immunoprecipitation revealed that glucocorticoid-transactivated TSC22D3 interacted with HIF-1α, leading to degradation of hypoxia-stabilized HIF-1α via the ubiquitin-proteasome pathway. Silencing TSC22D3 reversed glucocorticoid-mediated ubiquitination of HIF-1α and subsequent down-regulation of HIF-1α and galectin-1/LGALS1 levels. Glucocorticoid treatment to mice significantly alleviated diabetes-induce...Continue Reading

References

Oct 9, 1995·FEBS Letters·P SalvatoreL Chiariotti
May 8, 1997·Biochemical and Biophysical Research Communications·R NewtonP J Barnes
Feb 23, 2000·FEBS Letters·E MinetC Michiels
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jocelyn HolashJohn S Rudge
Apr 26, 2003·Investigative Ophthalmology & Visual Science·Susumu IshidaAnthony P Adamis
Apr 26, 2003·Investigative Ophthalmology & Visual Science·Kousuke NodaEiji Ikeda
Jun 18, 2003·The Journal of Biological Chemistry·Tsunenori KodamaHirotoshi Tanaka
Mar 20, 2004·Experimental & Molecular Medicine·Ji-Won LeeKyu-Won Kim
Jun 18, 2004·The Journal of Biological Chemistry·Tatsuya Kondo, C Ronald Kahn
Jul 15, 2006·Glycobiology·Isabelle CambyRobert Kiss
Nov 30, 2006·Journal of Cellular Biochemistry·Heidi H HauPaul R Bohjanen
Jun 15, 2007·Molecular and Cellular Endocrinology·Andrew R Clark
Aug 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Przemyslaw JuszczynskiMargaret A Shipp
Apr 18, 2009·Science·Sebastiaan H MeijsingKeith R Yamamoto
Apr 18, 2009·Seminars in Ophthalmology·Paolo S SilvaLloyd Paul Aiello
Jul 2, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Emira Ayroldi, Carlo Riccardi
Oct 23, 2010·Molecular Cell·Amar J MajmundarM Celeste Simon
Jan 5, 2011·British Journal of Pharmacology·Peter J Barnes
Feb 15, 2012·Annals of the New York Academy of Sciences·Fu-Tong LiuDaniel K Hsu
Sep 8, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Emira AyroldiCarlo Riccardi
Nov 26, 2015·Nucleic Acids Research·Jörg FallmannIvo L Hofacker
Dec 10, 2015·Scientific Reports·Atsuhiro KandaSusumu Ishida
Jan 19, 2018·The British Journal of Ophthalmology·Yoshihiro TakamuraUNKNOWN writing committee of Japan-Clinical Retina Research Team (J-CREST)
Oct 3, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Di WuSusumu Ishida

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Citations

May 29, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Hui-Yao LiXin-Yuan Gao

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Methods Mentioned

BETA
PCR
electrophoresis
Enzymatic Immunoprecipitation
ELISA
ubiquitination
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