PMID: 7914402Aug 1, 1994Paper

Glucocorticoids activate somatostatin gene transcription through co-operative interaction with the cyclic AMP signalling pathway

The Biochemical Journal
J L LiuY C Patel

Abstract

The somatostatin (SS) gene is transcriptionally regulated via the cyclic AMP (cAMP) response element (CRE), located in the proximal promoter (-41 to -48 bp). We have previously reported that glucocorticoids induce dose-dependent cell-specific alterations in the steady-state SS mRNA level. Here we have investigated direct transcriptional control of the SS gene by glucocorticoids. We have examined transcriptional interaction between glucocorticoids and the cAMP signalling pathway and mapped the 5' upstream regulatory region of the SS gene involved in glucocorticoid transactivation. Transcriptional regulation was determined by analysis of chloramphenicol acetyltransferase (CAT) activity in PC12 rat pheochromocytoma cells and A126-1B2 (protein kinase A-deficient mutant PC12) cells, by acute transfection of 5' flanking SS DNA (- 750, -250 and -71 bp) ligated to the reporter (CAT) gene. Dexamethasone (DEX) induced a dose-dependent 2.2-fold stimulation of SS gene transcription in PC12 cells, but not in A126-1B2 cells. Other steroid and thyroid hormones tested, and retinoic acid, were ineffective, while cAMP and forskolin stimulated gene transcription 4-5-fold in PC12 cells but not in A126-1B2 cells. DEX exerted an additive effect on c...Continue Reading

Citations

Dec 3, 2008·Brain Research·George A RoggeMichael J Kuhar
Aug 11, 2007·Molecular and Cellular Endocrinology·Olivier Kassel, Peter Herrlich
Sep 13, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·L A BachS L Leng
Nov 29, 2005·Molecular and Cellular Endocrinology·Kathleen Van CraenenbroeckGuy Haegeman
Apr 23, 2015·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Marianne Andersen
Aug 26, 2016·BMC Biotechnology·Betul BilginS Patrick Walton
Jun 8, 2006·Neuroscience·A M Hernández-PintoE Arilla-Ferreiro
May 2, 2003·Nature Reviews. Cancer·Loning Fu, Cheng Chi Lee
Jun 29, 2006·Pancreas·Nikiforos BallianXiao-Ping Wang
Jul 29, 1998·Endocrinology·Y J JengM S Soloff
Mar 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anthony N GerberMarc I Diamond
Dec 5, 1998·The American Journal of Physiology·M A Turman, C A Apple
May 7, 2021·Cellular and Molecular Gastroenterology and Hepatology·Noga KorenfeldIdo Goldstein

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