PMID: 9538932Apr 16, 1998Paper

Glucocorticoids and protein kinase C regulate neutral endopeptidase 24.11 in human vascular smooth muscle cells

Basic Research in Cardiology
K GrafG Kunkel

Abstract

Neutral endopeptidase 24.11 (NEP) degrades vasoactive peptides, including natriuretic peptides, kinins, angiotensins, and endothelins. It contributes to the regulation of vascular tone and body fluid homeostasis. In the present study the expression of NEP was investigated in cultured human smooth muscle cells derived from umbilical veins (HSMC) and human coronary arteries (HCSMC). A constitutive NEP expression was found in growing and starved smooth muscle cells and was about 4 fold higher than in endothelial cells derived from umbilical veins. Treatment of smooth muscle cells with dexamethasone (0.01-0.1 microM Dex) and with the protein kinase C activator, phorbol myristate acetate (0.1 microM PMA), increased NEP mRNA by 3-4 fold and two fold, respectively. Dexamethasone (0.1 microM) and prednisolone (0.1 microM) increased protein concentrations of NEP and NEP-activity after 3 days and continued to increase at 5 days, whereas PMA induced maximal increase of NEP concentrations after 48 hours. The effect of dexamethasone was concentration-dependent and was completely abolished by cycloheximide (10 microM), a protein synthesis inhibitor. The effect of PMA on NEP protein was completely blocked by protein kinase C inhibitors, calph...Continue Reading

Citations

Jan 8, 2009·Journal of Molecular Neuroscience : MN·Zhi-Min XiaoJian Huang
Feb 19, 2013·PloS One·Naoko MorisakiShigeru Moriwaki
Jul 31, 2012·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Ina CallebautPeter W Hellings
Jan 15, 2008·International Journal of Cardiology·Micha T MaederHans P Brunner-La Rocca
Aug 15, 2015·Molecular and Cellular Biochemistry·Barbara LewkoJan Stępiński
Apr 16, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K J PaunickaJ Y Niederkorn
Sep 30, 2010·The Journal of Biological Chemistry·Naoko MorisakiGenji Imokawa
May 21, 2014·Journal of Clinical and Experimental Neuropsychology·Nikita L van der ZwaluwLisette C P G M de Groot

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