PMID: 11321357Apr 26, 2001Paper

Glucocorticoids and TGF-beta1 synergize in augmenting fibroblast mediated contraction of collagen gels

Inflammation
F Q WenS I Rennard

Abstract

TGF-beta plays a central role in the initiation and progression of pulmonary fibrosis. Glucocorticoids are frequently used to treat fibrotic diseases, but beneficial effects are often modest. Both TGF-beta and glucocorticoids have been reported to increase fibroblast contraction of native collagen gels, a model of fibrotic tissue remodeling. Therefore, we sought to determine how glucocorticoids interact with TGF-beta in this system. In this study, human fetal lung fibroblasts (HFL-1) were pretreated with or without TGF-beta for 72 h before they were cast into type I collagen gels. Various concentrations of glucocorticoids (budesonide or hydrocortisone) were added at the time of casting. Gel size was then monitored at different times after gel release. The surrounding media were collected for the assay of prostaglandin E2 (PGE2) and the cell lysates were analyzed for cyclooxygenase (COX) expression by immunoblot. Glucocorticoids alone significantly enhanced fibroblast-mediated contraction of collagen gels (P < 0.01) and dose-dependently inhibited PGE2 release by HFL-1 fibroblasts. TGF-beta significantly augmented gel contraction but also induced a 30% increase in PGE2 release and increased the expression of COX-1. Glucocorticoid...Continue Reading

Citations

Apr 4, 2002·In Vitro Cellular & Developmental Biology. Animal·Robert B Vernon, Michel D Gooden
Aug 24, 2012·Journal of Translational Medicine·Oskar HallgrenGunilla Westergren-Thorsson
Nov 18, 2016·American Journal of Respiratory Cell and Molecular Biology·Christie A OjiakuReynold A Panettieri
Apr 17, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mark A Carlson, Michael T Longaker
Mar 24, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shinsaku TogoStephen I Rennard
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Feb 3, 2012·American Journal of Respiratory Cell and Molecular Biology·Masanori NakanishiStephen I Rennard
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Jun 9, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Tadashi KohyamaStephen I Rennard

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