PMID: 6113272Jun 1, 1981

Glucocorticoids increase tyrosine hydroxylase activity in cultured murine neuroblastoma

Journal of Neurochemistry
L WilliamsT H Williams

Abstract

Cyclic AMP and glucocorticoids appear to have a role in regulating the activity of tyrosine hydroxylase (TH), as well as the expression of "morphological differentiation" in murine neuroblastoma. Monolayer cultures of C-1300 murine neuroblastoma (clone NBP2) were treated with the following compounds in ethanol: dexamethasone, triamcinolone acetonide, hydrocortisone, cortexolone, androstenedione, testosterone, estradiol-17 beta; or with the phosphodiesterase inhibitor Ro20-1724 [4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone]. Treatment with either 200 micrograms/ml Ro20-1724 or 50 micrograms/ml dexamethasone produced significant increases in TH activity compared to alcohol controls (1.44 vs. 0.82 nmol 14CO2/mg protein/hr compared to 0.095). Triamcinolone acetonide or hydrocortisone also produced smaller, but significant, increases in TH activity compared to dexamethasone. When steroid activities were compared at 25 microM concentration and after 60 min of incubation (to maximize TH activity), triamcinolone acetonide was not as effective (62%) as dexamethasone. The relatively inactive glucocorticoid cortexolone produced a slight but significant increase, while the androgens androstenedione and testosterone and the estrogen estra...Continue Reading

References

Apr 25, 1977·Biochemical and Biophysical Research Communications·K NagaiahG Guroff
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T H JohD J Reis
Oct 15, 1979·Experimental Cell Research·D SandquistT H Williams
May 1, 1975·Biological Reviews of the Cambridge Philosophical Society·K N Prasad
Feb 1, 1974·Metabolism: Clinical and Experimental·E B Thompson, M E Lippman
Jan 1, 1974·Methods in Enzymology·A L Steiner
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J C WaymireK N Prasad

Citations

Jun 1, 1995·Journal of Pharmaceutical and Biomedical Analysis·M D Luque de CastroR Quiles
Mar 6, 2018·Frontiers in Pharmacology·Abeer K MalkawiAnas M Abdel Rahman
Sep 18, 2001·The Journal of Comparative Neurology·K A BerghornG E Hoffman
Oct 20, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Naohiko IkegakiXao X Tang

Related Concepts

Cyclic AMP, (R)-Isomer
Ethanol
Androstenedione
Cortodoxone
Oradexon
Estradiol, (16 alpha,17 beta)-Isomer
Glucocorticoid Effect
Neuroblastoma
4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone
Sterotate

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