Glucocorticoids mediate a decrease in AVP-regulated urea transporter in diabetic rat inner medulla

The American Journal of Physiology
J D KleinJ M Sands

Abstract

Providing glucocorticoids to adrenalectomized (Adx) rats results in downregulation of the vasopressin (AVP)-regulated urea transporter (VRUT) in the renal inner medullary (IM) tip. To examine the physiological relevance of this response, we studied rats with uncontrolled diabetes mellitus induced by streptozotocin (STZ), since these rats have increased corticosterone production and urea excretion. We measured VRUT protein in extracts from the IM tip or base of pair-fed control and diabetic rats by Western analysis using an antibody to rat VRUT. In the IM tip, VRUT was significantly reduced by 39% in diabetic compared with control rats. In the IM base, there was no significant difference between diabetic and control rats. To determine whether the decrease in VRUT in the IM tip was mediated by glucocorticoids, the experiment was repeated using the following three groups of rats: 1) Adx alone, 2) Adx + STZ, and 3) Adx + STZ + replacement with a physiological dose of glucocorticoid. There was no significant difference in VRUT between Adx and Adx + STZ rats. However, VRUT was significantly reduced by 32% in the IM tip of glucocorticoid-treated Adx + STZ rats compared with control Adx + STZ rats. We conclude that glucocorticoids regu...Continue Reading

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Citations

Nov 13, 2001·American Journal of Physiology. Renal Physiology·S CombetJ M Verbavatz
Feb 8, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Rudy M OrtizC Leo Ortiz
Aug 10, 2004·Current Opinion in Nephrology and Hypertension·Jeff M Sands
May 12, 2010·American Journal of Physiology. Cell Physiology·Abinash C MistryOtto Fröhlich
Dec 24, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·John J DoranJeff M Sands
Sep 24, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sun-Woo LimJin Kim
Jun 7, 2013·American Journal of Physiology. Renal Physiology·Benjamin KoRobert S Hoover
Dec 30, 2011·American Journal of Physiology. Renal Physiology·Titilayo O IloriJanet D Klein
Nov 8, 2014·American Journal of Physiology. Renal Physiology·Carol A HobanMitsi A Blount
Oct 29, 2000·American Journal of Physiology. Renal Physiology·A KatoJ M Sands
Jan 14, 2003·Annual Review of Physiology·Jeff M Sands
Jun 22, 2006·American Journal of Physiology. Renal Physiology·Janet D KleinJeff M Sands
Aug 10, 2007·American Journal of Physiology. Renal Physiology·Mitsi A BlountJeff M Sands
Sep 9, 2006·Journal of the American Society of Nephrology : JASN·Janet D KleinJeff M Sands
Aug 22, 2008·American Journal of Physiology. Renal Physiology·Nathan W BlessingJanet D Klein
Sep 8, 2001·American Journal of Physiology. Cell Physiology·R T TimmerJ M Sands
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Sophie CombetMarie-Marcelle Trinh-Trang-Tan
Apr 18, 2008·American Journal of Physiology. Renal Physiology·Mitsi A BlountJanet D Klein
Jun 12, 2009·American Journal of Physiology. Renal Physiology·Abinash C MistryOtto Fröhlich
Jul 15, 2004·American Journal of Physiology. Renal Physiology·Dongun KimJeff M Sands
Apr 6, 2002·American Journal of Physiology. Renal Physiology·Tao PengSerena M Bagnasco
Feb 24, 2001·American Journal of Physiology. Renal Physiology·J M TerrisJ B Wade
Apr 17, 2003·American Journal of Physiology. Renal Physiology·Dongun KimJanet D Klein
Oct 25, 2002·Journal of the American Society of Nephrology : JASN·Jeff M Sands
Feb 22, 2002·Journal of the American Society of Nephrology : JASN·Janet D KleinJeff M Sands
Dec 12, 2001·Kidney International·C A EcelbargerB K Kishore
Feb 19, 2002·Kidney International·Janet D KleinJeff M Sands
May 10, 2005·Biochimica Et Biophysica Acta·D T WardD Riccardi

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