PMID: 9164994May 1, 1997Paper

Glucocorticoids play an important role in mediating the enhanced metabolism of arginine and glutamine in enterocytes of postweaning pigs

The Journal of Nutrition
N E Flynn, G Wu

Abstract

Weaning is associated with increased intestinal metabolism of glutamine and arginine as well as elevated plasma concentrations of cortisol (the major circulating glucocorticoid) in pigs. The objective of this study was to determine if cortisol plays an important role in mediating the enhanced amino acid metabolism in enterocytes of weaned pigs by administering RU486 (a glucocorticoid receptor antagonist). Eighteen 21-d-old pigs were randomly assigned to three groups of six. Two of these groups received intramuscular injections of 0 or 10 mg RU486 per kg body weight 5 min before and 24 and 72 h after weaning to a corn-soybean meal-based diet. The third group was allowed to suckle freely from sows. When the pigs were 29 d old, jugular venous blood was obtained and pigs were killed for preparation of jejunal enterocytes. The activities of arginase, argininosuccinate synthase (ASS), argininosuccinate lyase (ASL) and pyrroline-5-carboxylate (P5C) synthase were measured. For metabolic studies, cells were incubated for 0 or 30 min at 37 degrees C in 2 mL of Krebs-bicarbonate buffer (pH 7.4) containing 0 or 2 mmol/L L-[U-14C]arginine or 2 mmol/L L-[U-14C]glutamine. In comparison with suckling pigs, weaning resulted in increases in the ...Continue Reading

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Citations

May 9, 2012·Animal : an International Journal of Animal Bioscience·P SuperchiA Sabbioni
Jul 27, 2000·American Journal of Physiology. Endocrinology and Metabolism·G WuD A Knabe
Aug 4, 1998·The Journal of Nutrition·G Wu
May 9, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·C Bouteloup-DemangeP Dechelotte
Feb 23, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Silvio NadalinMassimo Malagó
Jun 20, 2019·Animals : an Open Access Journal From MDPI·Qiangjun WangZhonghong Wu
Dec 1, 2009·Animal : an International Journal of Animal Bioscience·J P LallèsD Torrallardona
Aug 12, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·G WuL A Jaeger
Feb 26, 2011·Neuroscience and Biobehavioral Reviews·Canteros Maria Griselda

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