PMID: 2123388Oct 1, 1990Paper

Glucocorticoids regulate the amount of G proteins in rat aorta

Journal of Molecular Endocrinology
R M HaighG Milligan

Abstract

Glucocorticoids are known to influence cardiovascular sensitivity to catecholamines but the molecular mechanisms are undefined. We recently showed that glucocorticoids control the coupling of adrenergic receptors to G protein. Alterations in the amount of G protein is one mechanism by which receptor-G protein coupling may be controlled. Therefore, we set out to measure the levels of G proteins in aorta from normal, adrenalectomized and dexamethasone-treated adrenalectomized rats. G proteins were measured in plasma membrane preparations by immunoblotting and horseradish peroxidase staining. After adrenalectomy there was 53% (n = 5) decrease in the density of staining for Gi (ANOVA; P less than 0.05 compared to controls). Conversely, there was a 210% (n = 5) increase in the density of staining for Gs. The levels of Go and the beta-subunit of G proteins were not changed by adrenalectomy. Dexamethasone-replacement treatment after adrenalectomy returned Gi and Gs close to control values. Go remained unaltered compared to controls but was 24% (n = 3) less than the adrenalectomized values (ANOVA; P less than 0.05). The levels of beta-subunit after dexamethasone replacement were significantly greater (ANOVA; P less than 0.05) than both...Continue Reading

Citations

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