PMID: 9194766Jun 1, 1997Paper

Glucocorticoids stimulate cholesteryl ester formation in human smooth muscle cells

Arteriosclerosis, Thrombosis, and Vascular Biology
I PetrichenkoJ Larrue

Abstract

The aim of the present study was to investigate the effect of synthetic glucocorticoid dexamethasone (Dex) on cholesterol esterification in cultured human smooth muscle cells (SMC). In labeled SMC, Dex stimulated the esterification of [3H]cholesterol in a dose-dependent manner. This effect was specific for glucocorticoid hormones and could be inhibited by cycloheximide (3 ng/mL), actinomycin D (10(-5) mol/L), and the specific glucocorticoid antagonist RU 486 (10(-8) mol/L). When plasma membrane was selectively labeled with trace quantities of [3H]cholesterol (0.25 microCi/mL, 1 hour, 10 degrees C), Dex (10(-8) mol/L) caused a net flux of free [3H]cholesterol into the cells. Moreover, Dex (10(-8) mol/L, 24 hours) stimulated the esterification of sterols, newly synthesized from [14C]mevalonate (10 microCi/mL, 4 hours) and lowered the amount of [14C]sterols susceptible for cholesterol oxidase. The incorporation of [14C]oleic acid into cholesteryl esters was markedly higher in Dex-pretreated SMC than in the control cells (2.1 +/- 0.07 and 1.4 +/- 0.1 pmol/h/microgram protein, respectively, P < .01). At the time, cholesteryl ester hydrolysis in Dex-treated cells was reduced (72 +/- 8 pmol cholesteryl esters/h per milligram versus 13...Continue Reading

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Citations

Dec 16, 2003·The Journal of Steroid Biochemistry and Molecular Biology·S JindrichováJ Pácha
Feb 22, 2012·Cardiovascular Research·Sima AllahverdianGordon A Francis
Nov 10, 2009·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·M E HolmesJ C Eisenmann
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Jul 25, 2021·International Journal of Molecular Sciences·Clare MacLeodMark Nixon
Aug 5, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yu-Xiao LiuBo Hu

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