Abstract
Glucocorticosteroids (GCS) are beneficial in allergic asthma. GCS therapy results in reduced mRNA expression of interleukin-4 (IL-4) and IL-5 in cells from bronchoalveolar lavage (BAL) but not of IFN-gamma. In vitro studies with blood-derived T cells, however, show inhibition of all three cytokines by GCS. We studied the effects of GCS on T cells from BAL in vitro, namely Th0-, Th1, and Th2-like clones; and we compared BAL- with blood-derived clones. Dexamethasone (DEX) inhibited the anti-CD3-induced production of IL-4, IL-5 and IFN-gamma in all 20 clones tested. IFN-gamma production was inhibited significantly less than IL-4 and IL-5. DEX enhanced the ratio IFN-gamma/IL-4 (mean +/- SEM: control, 28.7 +/- 17.6; with 10-7 M DEX, 55.0 +/- 27.5, P<0.005). Interestingly, two categories of clones were distinguished based on the effects of GCS on IL-2 production and IL-2R alpha expression and proliferation; 1) In low IL-2 producers DEX blocked IL-2 production and decreased IL-2R alpha expression and proliferation; 2) In high IL-2 producers DEX inhibited IL-2 production partially and enhanced IL-2R alpha expression and proliferation. Anti-IL-2 and anti-IL2R alpha blocked the DEX-induced increase in proliferation. High levels of added ...Continue Reading
Citations
Mar 29, 2002·Hematological Oncology·Wassim Y AlmawiXian C Li
Oct 24, 1997·The Journal of Allergy and Clinical Immunology·S P UmlandM M Billah
Jul 1, 1998·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·I C CrockerR G Townley
Oct 9, 1999·Clinical Transplantation·W Y AlmawiM J Rieder
Apr 27, 1999·British Journal of Pharmacology·A Z El-HashimC P Page
Aug 11, 1998·Clinical and Experimental Immunology·S HodgeP Han
Oct 29, 1998·Mediators of Inflammation·V H van der Velden
Oct 27, 1998·Allergy·F M Cuss
Apr 1, 1998·American Journal of Respiratory and Critical Care Medicine·P J BarnesW W Busse
Sep 16, 2000·American Journal of Respiratory and Critical Care Medicine·C MarguetJ O Warner
Sep 16, 2000·American Journal of Respiratory and Critical Care Medicine·E A KellyN N Jarjour
Sep 11, 2001·American Journal of Respiratory and Critical Care Medicine·K KurashimaS Nakao
May 7, 2002·American Journal of Respiratory and Critical Care Medicine·Sukhbir K ShahidPeter J Barnes
Jun 11, 2011·Cytokine·Manoj LamaTapas Kumar Chaudhuri
Dec 17, 2014·Parasites & Vectors·Vitor Luís Tenório MatiAlan Lane de Melo
Jun 10, 2005·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Anneke M LandstraDirkje S Postma
Apr 2, 1998·The Journal of Laboratory and Clinical Medicine·P S MuraliP A Greenberger
Dec 17, 2005·Pulmonary Pharmacology & Therapeutics·Fabio L M Ricciardolo
Mar 12, 1999·Annual Review of Medicine·B A Cockrill, C A Hales
Sep 2, 1999·Tropical Medicine & International Health : TM & IH·G A Rook, R Baker
Jan 1, 2006·Expert Review of Clinical Immunology·Marc Williams, Steve Georas
Feb 9, 2006·Respiration; International Review of Thoracic Diseases·Sachiko KaiKiyohisa Sekizawa
Mar 22, 2006·Respirology : Official Journal of the Asian Pacific Society of Respirology·Kazuyoshi KurashimaKouji Matsushima
May 10, 2002·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Senja KannistoMatti Korppi
Aug 24, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M E Lachowicz-ScrogginsH A Boushey