PMID: 15331544Aug 28, 2004Paper

Glucokinase is an integral component of the insulin granules in glucose-responsive insulin secretory cells and does not translocate during glucose stimulation

Diabetes
Catherine ArdenLoranne Agius

Abstract

The association of glucokinase with insulin secretory granules has been shown by cell microscopy techniques. We used MIN6 insulin-secretory cells and organelle fractionation to determine the effects of glucose on the subcellular distribution of glucokinase. After permeabilization with digitonin, 50% of total glucokinase remained bound intracellularly, while 30% was associated with the 13,000g particulate fraction. After density gradient fractionation of the organelles, immunoreactive glucokinase was distributed approximately equally between dense insulin granules and low-density organelles that cofractionate with mitochondria. Although MIN6 cells show glucose-responsive insulin secretion, glucokinase association with the granules and low-density organelles was not affected by glucose. Subfractionation of the insulin granule components by hypotonic lysis followed by sucrose gradient centrifugation showed that glucokinase colocalized with the granule membrane marker phogrin and not with insulin. PFK2 (6-phosphofructo-2-kinase-2/fructose-2,6-bisphosphatase)/FDPase-2, a glucokinase-binding protein, and glyceraldehyde phosphate dehydrogenase, which has been implicated in granule fusion, also colocalized with glucokinase after hypoto...Continue Reading

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Citations

Aug 30, 2008·Cellular and Molecular Life Sciences : CMLS·P B Iynedjian
Aug 20, 2012·Journal of Diabetes Investigation·Ronald Dirkx, Michele Solimena
Mar 18, 2006·FEBS Letters·Catherine ArdenLoranne Agius
Sep 13, 2011·Progress in Biophysics and Molecular Biology·Jae-Hyung ParkDae-Kyu Song
Apr 29, 2005·Diabetes/metabolism Research and Reviews
Jul 24, 2012·Biochimica Et Biophysica Acta·Maria NegahdarLise Bjørkhaug
Nov 26, 2005·Biochimica Et Biophysica Acta·John J Lemasters, Ekhson Holmuhamedov
Aug 25, 2016·Journal of Clinical Laboratory Analysis·Mauren Isfer Anghebem-OliveiraFabiane Gomes de Moraes Rego
Sep 15, 2016·International Journal of Molecular Sciences·Milou Groot NibbelinkAart Van Apeldoorn
Feb 24, 2007·Molecular & Cellular Proteomics : MCP·Yannick BrunnerJean-Charles Sanchez
Aug 22, 2017·Pharmacological Reports : PR·Ashwini KumarAwanish Kumar
Nov 5, 2013·The Journal of Biological Chemistry·Anke Hofmeister-BrixSimone Baltrusch
Jan 23, 2021·International Journal of Molecular Sciences·Elisa MascoloFiammetta Vernì

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