Glucolipotoxicity in INS-1E cells is counteracted by carnitine palmitoyltransferase 1 over-expression

Biochemical and Biophysical Research Communications
E Maria SolPeter Bergsten

Abstract

Effects of non-esterified fatty acids (FAs) are accentuated when applied together with elevated glucose through preferential use of glucose as fuel, which leads to decreased oxidation of FAs. We examined how over-expression of the mitochondrial FA transporter carnitine palmitoyltransferase 1 (CPT1) affects glucose-stimulated insulin secretion (GSIS), apoptosis and ER stress in INS-1E cells cultured in the presence of elevated levels of glucose and palmitate. INS-1E cells were infected with Tet-ON regulated adenovirus containing CPT1 and cultured for 48h in the presence of 0.5mM palmitate and 20mM glucose. Over-expressing CPT1 lowered basal insulin secretion in a dose-dependent manner thereby improving GSIS from INS-1E cells. Also, apoptosis was alleviated and ER-stress markers p-eIF2alpha and CHOP were decreased in cells over-expressing CPT1. We conclude that regulated over-expression of CPT1 is beneficial for glucolipotoxic beta-cells.

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Citations

Jul 13, 2011·Lipids in Health and Disease·Ernest Sargsyan, Peter Bergsten
Mar 18, 2015·Molecular and Cellular Endocrinology·Ik-Rak JungYup Kang
Mar 27, 2012·Annual Review of Biochemistry·Sung Hoon Back, Randal J Kaufman
Sep 17, 2013·Endocrinology·Hjalti KristinssonErnest Sargsyan
Jan 5, 2012·American Journal of Physiology. Endocrinology and Metabolism·Masaki KobayashiTadahiro Kitamura
Aug 24, 2018·BMC Genomics·Ernest SargsyanPeter Bergsten
Jan 27, 2011·American Journal of Physiology. Endocrinology and Metabolism·Christopher D Green, L Karl Olson
Jan 11, 2020·Molecular Aspects of Medicine·Guy LasOrian S Shirihai
Mar 10, 2021·Journal of Assisted Reproduction and Genetics·Qingwen RenWeiwei Wu

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