GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase

Plant Physiology
Toshiki IshikawaJenny C Mortimer

Abstract

Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane. Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability. However, the function of GIPCs and the significance of their structural variation are poorly understood. Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)]. In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants. However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal. Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup. This ...Continue Reading

Citations

Aug 29, 2020·Plant Signaling & Behavior·J A DavisJ F Harper
Jun 19, 2019·The New Phytologist·Eloïse HubySandrine Dhondt-Cordelier
May 15, 2020·Trends in Plant Science·Jenny C Mortimer, Henrik Vibe Scheller
Apr 1, 2021·The Journal of Biological Chemistry·Adiilah Mamode CassimSébastien Mongrand
Nov 23, 2018·Progress in Lipid Research·Adiilah Mamode CassimSébastien Mongrand
Jun 3, 2021·Biomolecules·Tibo De ConinckEls J M Van Damme
Jan 14, 2021·Plant & Cell Physiology·Jenny Mortimer
Nov 19, 2020·Chemistry and Physics of Lipids·Yi-Ting TsaiItay Budin

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