Glucose-6-phosphate dehydrogenase in blunt snout bream Megalobrama amblycephala: molecular characterization, tissue distribution, and the responsiveness to dietary carbohydrate levels

Fish Physiology and Biochemistry
Guang-Zhen JiangXiang-Fei Li

Abstract

This study aimed to characterize the full-length cDNA of glucose-6-phosphate dehydrogenase (G6PD) from Megalobrama amblycephala with its responses to dietary carbohydrate levels characterized. The cDNA obtained covered 2768 bp with an open reading frame of 1572 bp. Sequence alignment and phylogenetic analysis revealed a high degree of conservation (77-97%) among most fish and other higher vertebrates. The highest transcription of G6PD was observed in kidney followed by liver, whereas relatively low abundance was detected in eye. Then, the transcriptions and activities of G6PD as well as lipid contents were determined in the liver, muscle, and the adipose tissue of fish fed two dietary carbohydrate levels (30 and 42%) for 12 weeks. Hepatic transcriptions of fatty acid synthetase (FAS), acetyl-CoA carboxylase α (ACCα), sterol regulatory element-binding protein-1 (SREBP1), and peroxisome proliferator-activated receptor γ (PPARγ) were also measured to corroborate the lipogenesis derived from carbohydrates. The G6PD expressions and activities in both liver and the adipose tissue as well as the lipid contents in whole-body, liver, and the adipose tissue all increased significantly after high-carbohydrate feeding. Hepatic transcriptio...Continue Reading

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Citations

Aug 4, 2020·Critical Reviews in Analytical Chemistry·Barbora LegerskáStanislav Miertuš

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Datasets Mentioned

BETA
KF918747.1
KJ743994
CAA39089.1
AII80539.1

Methods Mentioned

BETA
electrophoresis
PCR
PCRs
acetylation

Software Mentioned

SeqMan II
Primer
pI / Mw
MEGA
SPSS
ClustalW
DNAStar Package
MegAlign

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