Glucose-6-phosphate dehydrogenase is a regulator of vascular smooth muscle contraction.

Antioxidants & Redox Signaling
Rakhee S GupteSachin A Gupte

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme in the pentose phosphate pathway and a major source of nicotinamide adenine dinucleotide phosphate reduced (NADPH), which regulates numerous enzymatic (including glutathione reductase and NADPH oxidase that, respectively, generates reduced glutathione and reactive oxygen species) reactions involved in various cellular actions, yet its physiological function is seldom investigated. We, however, recently showed that inhibiting G6PD causes precontracted coronary artery (CA) to relax in an endothelium-derived relaxing factor- and second messenger-independent manner. Here we assessed the role of G6PD in regulating CA contractility. Treating bovine CAs for 20 min with potassium chloride (KCl; 30 mM), amphotericin B (50 μM), or U46619 (100 nM) significantly (p < 0.05) increased both G6PD activity and glucose flux through the pentose phosphate pathway. The effect was Ca(2+) independent, and there was a corresponding increase in protein kinase C (PKC) activity. Activation of G6PD by KCl was blocked by the PKCδ inhibitor rottlerin (10 μM) or by knocking down PKCδ expression using siRNA. Phorbol 12, 13-dibutyrate (10 μM), a PKC activator, significantly increased G6PD pho...Continue Reading

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Citations

Dec 18, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Peter A HeckerWilliam C Stanley
Mar 15, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Hirotaka AtaSachin A Gupte
May 15, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sukrutha ChettimadaSachin A Gupte
Jun 24, 2017·Antioxidants & Redox Signaling·Angelo D'AlessandroKurt R Stenmark
Dec 21, 2018·PloS One·Susama ChokesuwattanaskulHelen L Wright
Jan 1, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dhara PatelMichael S Wolin
May 23, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Rakhee GupteSachin A Gupte
Aug 9, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Jia ShiFan He
May 28, 2019·Journal of Cellular Biochemistry·Bor Luen Tang
Jan 9, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Vidhi DhagiaSachin A Gupte
May 20, 2021·Oxidative Medicine and Cellular Longevity·Maria Pina DoreGiovanni Mario Pes

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Methods Mentioned

BETA
transfection
electrophoresis
Immunoprecipitation
pull-down
coimmunoprecipitation
confocal microscopy
transfecting

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