Glucose-6-phosphate dehydrogenase is indispensable in embryonic development by modulation of epithelial-mesenchymal transition via the NOX/Smad3/miR-200b axis

Cell Death & Disease
Yi-Hsuan WuDaniel Tsun-Yee Chiu

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme involved in the pentose phosphate shunt for producing nicotinamide adenine dinucleotide phosphate (NADPH). Severe G6PD deficiency leads to embryonic lethality, but the underlying mechanism is unclear. In the current study, the effects of G6PD on epithelial-mesenchymal transition (EMT), especially during embryonic development, were investigated. The knockdown of G6PD induced morphological changes, accompanied by the suppression of epithelial markers, E-cadherin and β-catenin, in A549 and MDCK cells. Such modulation of EMT was corroborated by the enhancement of migration ability in G6PD-knockdown A549 cells. Zebrafish embryos with g6pd knockdown exhibited downregulation of the E-cadherin/β-catenin adhesion molecules and impaired embryonic development through reduction in epiboly rate and increase in cell shedding at the embryo surface. The dysregulation in zebrafish embryonic development caused by g6pd knockdown could be rescued through human G6PD or CDH1 (E-cadherin gene) cRNA coinjection. The Smad3/miR-200b axis was dysregulated upon G6PD knockdown, and the reconstitution of SMAD3 in G6PD-knockdown A549 cells restored the expression of E-cadherin/β-catenin. The i...Continue Reading

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Citations

Sep 11, 2019·Cells·Hung-Chi YangDaniel Tsun-Yee Chiu
Jun 30, 2018·Journal of Experimental & Clinical Cancer Research : CR·Jing ChenQian Wang
Jul 14, 2020·Oxidative Medicine and Cellular Longevity·Hoda MoazzenQingping Feng
Jul 28, 2020·Journal of Fungi·Kamel A Abd-ElsalamAlexander Yu Vasil'kov
Nov 9, 2018·Antioxidants·Christine RamponSophie Vriz
Sep 26, 2020·Biomolecules·Maximilian Breuer, Shunmoogum A Patten
Jan 7, 2021·Free Radical Research·Hung-Chi YangDaniel Tsun-Yee Chiu
Dec 10, 2020·Seminars in Perinatology·Ronald J WongVinod K Bhutani
Mar 17, 2021·Cell Death & Disease·Xiaoyu XuZeper Abliz

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Methods Mentioned

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PCR
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Software Mentioned

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