Glucose Acutely Reduces Cytosolic and Mitochondrial H2 O2 in Rat Pancreatic Beta Cells

Antioxidants & Redox Signaling
Jean-Philippe DeglasseJean-Christophe Jonas

Abstract

Aims: Whether H2O2 contributes to the glucose-dependent stimulation of insulin secretion (GSIS) by pancreatic β cells is highly controversial. We used two H2O2-sensitive probes, roGFP2-Orp1 (reduction/oxidation-sensitive enhanced green fluorescent protein fused to oxidant receptor peroxidase 1) and HyPer (hydrogen peroxide sensor) with its pH-control SypHer, to test the acute effects of glucose, monomethyl succinate, leucine with glutamine, and α-ketoisocaproate on β cell cytosolic and mitochondrial H2O2 concentrations. We then tested the effects of low H2O2 and menadione concentrations on insulin secretion. Results: RoGFP2-Orp1 was more sensitive than HyPer to H2O2 (response at 2-5 vs. 10 μM) and less pH-sensitive. Under control conditions, stimulation with glucose reduced mitochondrial roGFP2-Orp1 oxidation without affecting cytosolic roGFP2-Orp1 and HyPer fluorescence ratios, except for the pH-dependent effects on HyPer. However, stimulation with glucose decreased the oxidation of both cytosolic probes by 15 μM exogenous H2O2. The glucose effects were not affected by overexpression of catalase, mitochondrial catalase, or superoxide dismutase 1 and 2. They followed the increase in NAD(P)H autofluorescence, were maximal at ...Continue Reading

References

Feb 1, 1987·The Journal of Experimental Medicine·J O'Donnell-TormeyJ de la Harpe
Jan 7, 2003·The Journal of Biological Chemistry·Vytautas P BindokasLouis H Philipson
Apr 22, 2004·American Journal of Physiology. Endocrinology and Metabolism·M Z KhaldiJ C Jonas
Mar 19, 2005·The Journal of Biological Chemistry·Geert A MartensDaniel Pipeleers
Mar 24, 2006·Nature Methods·Vsevolod V BelousovSergey Lukyanov
Jan 24, 2009·The EMBO Journal·Andreas WiederkehrClaes B Wollheim
Mar 21, 2009·Molecular Endocrinology·Thomas M ReinbotheErik Renström
Jun 9, 2009·Toxicology and Applied Pharmacology·Jingbo PiMelvin E Andersen
Jan 22, 2010·Antioxidants & Redox Signaling·Andreas J Meyer, Tobias P Dick
Aug 10, 2010·Bioorganic & Medicinal Chemistry·Kseniya N MarkvichevaVsevolod V Belousov
Jan 13, 2011·The Journal of Biological Chemistry·Damon PoburkoNicolas Demaurex
Jul 26, 2011·Molecular and Cellular Endocrinology·Andreas Wiederkehr, Claes B Wollheim
Oct 4, 2011·Free Radical Biology & Medicine·Bruce MorganTobias P Dick
Nov 9, 2011·Free Radical Biology & Medicine·Alexandre SarreFrançoise Assimacopoulos-Jeannet
Jun 25, 2013·Cell Metabolism·Marc PrentkiS R Murthy Madiraju
Apr 1, 2014·The Biochemical Journal·Hilton K TakahashiJean-Christophe Jonas
Nov 18, 2014·Nature Chemical Biology·Mirko C SobottaTobias P Dick
Sep 22, 2015·The Journal of Clinical Investigation·Mourad FerdaoussiPatrick E MacDonald
Feb 16, 2016·Nature Methods·William D CameronJonathan V Rocheleau
Mar 17, 2016·Science Signaling·Yuuta FujikawaTobias P Dick
Apr 19, 2016·Nature Chemical Biology·Bruce MorganTobias P Dick
Sep 25, 2016·Molecular and Cellular Endocrinology·Arnaldo H de SouzaJean-Christophe Jonas
Oct 13, 2016·The Biochemical Journal·Adam NealIan R Sweet
Dec 13, 2016·Free Radical Biology & Medicine·Dmitry S Bilan, Vsevolod V Belousov
Mar 1, 2017·Free Radical Biology & Medicine·Alexandra MüllerLars I Leichert
Jun 8, 2017·Antioxidants & Redox Signaling·Sarah StöckerTobias P Dick
Nov 22, 2017·Antioxidants & Redox Signaling·Leticia Prates RomaBruce Morgan

❮ Previous
Next ❯

Citations

May 2, 2020·Redox Report : Communications in Free Radical Research·Gabriela Nunes Marsiglio-LibraisAngelo Rafael Carpinelli
Mar 19, 2020·EMBO Molecular Medicine·Sana S HasanAndreas Fischer
Apr 11, 2020·Pharmacological Reports : PR·Eloisa Aparecida Vilas-BoasLeticia Prates Roma
Dec 13, 2019·Oxidative Medicine and Cellular Longevity·Johanna SchultheisMartina Düfer
Oct 22, 2019·Journal of Molecular Biology·Leticia P Roma, Jean-Christophe Jonas

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
Assay
density gradient centrifugation

Software Mentioned

SypHer
ImageJ
HyPer
AdEasy System

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.