Glucose affects monocarboxylate cotransporter (MCT) 1 expression during mouse preimplantation development

Reproduction : the Official Journal of the Society for the Study of Fertility
Sarah JansenPeter L Kaye

Abstract

Cleavage-stage embryos have an absolute requirement for pyruvate and lactate, but as the morula compacts, it switches to glucose as the preferred energy source to fuel glycolysis. Substrates such as glucose, amino acids, and lactate are moved into and out of cells by facilitated diffusion. In the case of lactate and pyruvate, this occurs via H+-monocarboxylate cotransporter (MCT) proteins. To clarify the role of MCT in development, transport characteristics for DL-lactate were examined, as were mRNA expression and protein localisation for MCT1 and MCT3, using confocal laser scanning immunofluorescence in freshly collected and cultured embryos. Blastocysts demonstrated significantly higher affinity for DL-lactate than zygotes (Km 20 +/- 10 vs 87 +/- 35 mmol lactate/l; P = 0.03 by linear regression) but was similar for all stages. For embryos derived in vivo and those cultured with glucose, MCT1 mRNA was present throughout preimplantation development, protein immunoreactivity appearing diffuse throughout the cytoplasm with brightest intensity in the outer cortical region of blastomeres. In expanding blastocysts, MCT1 became more prominent in the cytoplasmic cortex of blastomeres, with brightest intensity in the polar trophectoder...Continue Reading

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Citations

May 13, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Jennifer SmithJonathan R Hill
Mar 13, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Henry J Leese
Jun 6, 2009·Reproductive Biomedicine Online·Jason E Swain, Thomas B Pool
Oct 9, 2009·Trends in Endocrinology and Metabolism : TEM·Scott H Purcell, Kelle H Moley
Mar 7, 2014·Reproductive Toxicology·Robert G Ellis-HutchingsEdward W Carney
Aug 27, 2013·Seminars in Oncology·Ubaldo E Martinez-OutschoornMichael P Lisanti
Jul 20, 2017·Molecular Reproduction and Development·Kailun Hu, Yang Yu

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