Glucose-dependent insulinotropic polypeptide (GIP) stimulates transepithelial glucose transport

Obesity
Satish K SinghM Michael Wolfe

Abstract

The purpose of this study was to characterize the effects of glucose-dependent insulinotropic peptide (GIP) on small intestinal glucose transport in vitro. Stripped proximal jejunum from fasted mice was mounted in Ussing chambers. The serosal side was bathed in Regular Ringer solution containing 5 mmol/l glucose, and the mucosal side, with solution containing 10 mmol/l 3-O-methyl glucose (3OMG). Intercellular cyclic adenosine monophosphate (cAMP), mucosa-to-serosa fluxes of 3OMG (J(ms)(3OMG)), and short-circuit current (I(SC)) were measured in the presence and absence of GIP. GIP increased cAMP by 2.5-fold in isolated enterocytes, consistent with a direct effect of GIP on these epithelial cells. GIP also increased I(SC) and J(ms)(3OMG) by 68 and 53%, respectively, indicating that the increase in J(ms)(3OMG) was primarily electrogenic, with a small electroneutral component. The stimulatory effect of GIP on J(ms)(3OMG) was concentration dependent. In addition, 1,000 nmol/l and 10 nmol/l GIP increased J(ms)(3OMG) by 70 and 30% over control, respectively, consistent with receptor activation. Phlorizin (20 mumol/l), an inhibitor of Na(+)-glucose cotransporter (SGLT-1), abolished the increase in I(SC) and decreased J(ms)(3OMG) by app...Continue Reading

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Citations

Apr 1, 2011·The Proceedings of the Nutrition Society·S P Shirazi-BeecheyM Al-Rammahi
Jul 15, 2011·European Journal of Endocrinology·Farid SaadLouis Gooren
Nov 26, 2010·Biochemical and Biophysical Research Communications·Eiichi OgawaNobuya Inagaki
Jun 30, 2016·Neuroscience·Masafumi JyotakiYuzo Ninomiya
Dec 6, 2018·Nutrition Reviews·Maude Le GallJohanne Le Beyec
Mar 11, 2010·Journal of Computer-aided Molecular Design·Carolina Horta AndradeAnton J Hopfinger
May 16, 2014·Journal of Clinical Gastroenterology·M Michael Wolfe, Michael O Boylan
Sep 28, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Steven D CoonSatish K Singh
Jan 25, 2018·Diabetes, Obesity & Metabolism·Michael A Nauck, Juris J Meier
Sep 11, 2019·The Journal of Nutrition·Heather A McCauley
Oct 22, 2015·American Journal of Physiology. Endocrinology and Metabolism·Michael O BoylanM Michael Wolfe
Nov 8, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Steven D CoonSatish K Singh
Dec 20, 2018·Frontiers in Endocrinology·Denise KreuchRichard L Young

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