Glucose-dependent insulinotropic polypeptide (GIP) induces calcitonin gene-related peptide (CGRP)-I and procalcitonin (Pro-CT) production in human adipocytes

The Journal of Clinical Endocrinology and Metabolism
Katharina TimperMirjam Christ-Crain

Abstract

Increased plasma levels of glucose-dependent insulinotropic polypeptide (GIP), calcitonin CT gene-related peptide (CGRP)-I, and procalcitonin (Pro-CT) are associated with obesity. Adipocytes express functional GIP receptors and the CT peptides Pro-CT and CGRP-I. However, a link between GIP and CT peptides has not been studied yet. The objective of the study was the assessment of the GIP effect on the expression and secretion of CGRP-I and Pro-CT in human adipocytes, CGRP-I and CT gene expression in adipose tissue (AT) from obese vs. lean subjects, and plasma levels of CGRP-I and Pro-CT after a high-fat meal in obese patients. Human preadipocyte-derived adipocytes, differentiated in vitro, were treated with GIP. mRNA expression and protein secretion of CGRP-I and Pro-CT were measured. Human CGRP-I and CT mRNA expression in AT and CGRP-I and Pro-CT plasma concentrations were assessed. Treatment with 1 nm GIP induced CGRP-I mRNA expression 6.9 ± 1.0-fold (P < 0.001 vs. control) after 2 h and CT gene expression 14.0 ± 1.7-fold (P < 0.001 vs. control) after 6 h. GIP stimulated CGRP-I secretion 1.7 ± 0.2-fold (P < 0.05 vs. control) after 1 h. In AT samples of obese subjects, CGRP-I mRNA expression was higher in sc AT (P < 0.05 vs. le...Continue Reading

References

Jul 1, 1991·Peptides·P M ZelissenW H Hackeng
Oct 16, 1989·Biochemical and Biophysical Research Communications·D KreutterK M Andrews
Oct 1, 1988·Regulatory Peptides·M Pettersson, B Ahrén
Dec 1, 1994·The Journal of Clinical Endocrinology and Metabolism·P DandonaC Bohuon
Oct 1, 1996·Scandinavian Journal of Clinical and Laboratory Investigation·U Pedersen-BjergaardN J Christensen
Jun 18, 2002·Nature Medicine·Kazumasa MiyawakiYutaka Seino
Aug 18, 2005·The Journal of Clinical Endocrinology and Metabolism·Jardena J PuderBeat Müller
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Christopher H S McIntosh
Sep 10, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·R E WeaverA J Balmforth
Mar 3, 2009·Vitamins and Hormones·Christopher H S McIntoshSu-Jin Kim
Apr 13, 2010·Diabetes, Obesity & Metabolism·M FischerJ Grisouard
Jun 11, 2010·The Journal of Clinical Endocrinology and Metabolism·Ali AbbasiStephan J L Bakker

❮ Previous
Next ❯

Citations

Aug 6, 2011·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·E PaschettaG Musso
Dec 15, 2011·Orvosi hetilap·Gabor Winkler
Oct 25, 2012·American Journal of Physiology. Endocrinology and Metabolism·Katharina TimperMirjam Christ-Crain
Sep 11, 2019·Cardiovascular Research·Wenqun LiYuanjian Li
Sep 9, 2017·Endocrine·William Gustavo LimaValéria Ernestânia Chaves
Dec 9, 2020·Molecular Metabolism·Jonathan E Campbell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.