Glucose deprivation increases basal and electrically evoked transmitter release from rat striatal slices. Role of NMDA and adenosine A1 receptors

European Journal of Pharmacology
S Jin, B B Fredholm

Abstract

We have investigated how glucose deprivation in vitro influences the basal and electrically evoked release of dopamine and acetylcholine from rat striatal slices and the role of endogenous activation of NMDA receptors and adenosine A1 receptors in determining the magnitude of this response. Rat striatal slices, preincubated with [3H]dopamine and [14C]choline, were superfused continuously and stimulated electrically. Before and during the second stimulation, some slices were superfused with glucose-free Krebs' solution. Such glucose deprivation caused a 2 to 3-fold increase of the electrically evoked, calcium-dependent release of endogenous adenosine (but not hypoxanthine and inosine) and [3H]dopamine and a 30% increase in release of [14C]acetylcholine. Glucose deprivation also caused a delayed increase in the release of [3H]dopamine, but not of [14C]acetylcholine. The dopamine release was not calcium dependent. The addition of 8-cyclopentyl-1,3-dipropylxanthine (DPCPX, 1 microM), a selective adenosine A1 receptor antagonist, slightly enhanced the glucose deprivation-induced stimulatory effect on the evoked release of these two transmitters, whereas the NMDA receptor antagonist dizocilpine((+)-5-methyl-10,11-dihydro-5H-dibenzo[a...Continue Reading

References

Dec 1, 1992·Trends in Pharmacological Sciences·K A RudolphiB B Fredholm
Apr 1, 1992·Trends in Neurosciences·C R Gerfen
Jan 1, 1987·Stroke; a Journal of Cerebral Circulation·T BrannanH Maker
Dec 1, 1974·British Journal of Pharmacology·I W Richardson, J C Szerb
Jan 1, 1984·Journal of Receptor Research·P J Marangos
Oct 1, 1995·British Journal of Pharmacology·M D CroningN R Newberry

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