PMID: 11927383Apr 3, 2002Paper

Glucose-inducible hypertrophy and suppression of anion efflux in rat beta cells

The Journal of Endocrinology
C B ChanR M MacPhail

Abstract

Hypertrophy of beta cells from obese fa/fa rats is associated with increased sensitivity to basal glucose. Exposure to glucose in culture distorts insulin secretion more in beta cells from large than small islets from fa/fa rats. The aim of the present study is to investigate whether increased beta cell volume is associated with both glucose hypersensitivity and altered activity of the glucose-sensitive anion conductance. Beta cells from fa/fa rats had increased volume compared with those from lean rats after 24 h culture. Three-day exposure to 25 mM glucose in culture induced 10-15% hypertrophy in beta cells from lean rats and basal secretion from intact islets was increased tenfold. Estimates of ion channel activity were made from measurement of radiolabeled ion efflux. Taurine efflux, a marker of glucose-regulated anion channel activity, was reduced after high glucose exposure but no alterations in glucose-dependent K+ efflux were detected. The reverse hemolytic plaque assay was used to determine the contributions of the number of secreting cells (recruitment) versus secretion per cell in beta cells from enlarged (>250 microm diameter), intermediate (125-250 microm) and small (<125 microm) islets from lean and obese rats exp...Continue Reading

Citations

May 31, 2007·The Journal of General Physiology·Tobias RoseMarjan Rupnik
Feb 15, 2008·Diabetes/metabolism Research and Reviews·Monique C SalehCatherine B Chan
Nov 24, 2005·American Journal of Physiology. Endocrinology and Metabolism·R R MacGregorL Stehno-Bittel

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