Glucose is necessary for stabilization of hypoxia-inducible factor-1alpha under hypoxia: contribution of the pentose phosphate pathway to this stabilization

FEBS Letters
Mayuko Osada-OkaTakashi Sato

Abstract

In this study, we observed that low glucose or fructose reduces the increase in hypoxia-inducible factor-1alpha (HIF-1alpha) protein under hypoxic conditions. 6-Aminonicotinamide (6-AN), an inhibitor of the pentose phosphate pathway (PPP), also inhibited the increase of HIF-1alpha protein under hypoxic conditions, while the reduced protein levels of HIF-1alpha by low glucose were apparently recovered by the addition of MG-132 or NADPH. Moreover, siRNA for glucose-6-phosphate dehydrogenase, which produces NADPH, reduced the increase in HIF-1alpha protein. On the other hand, cobalt-induced expression of HIF-1alpha protein was not affected by low glucose or 6-AN under normoxic conditions. In conclusion, glucose metabolism through the PPP, but not in glycolysis, plays an important role in the stabilization of HIF-1alpha protein under hypoxic conditions.

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Citations

Jan 10, 2016·Neurochemistry International·Sviatlana V HrynevichSergei V Fedorovich
Apr 5, 2013·Evolution; International Journal of Organic Evolution·James H MardenChristopher W Wheat
Jun 17, 2016·Molecular & Cellular Oncology·Kurosh Ameri, Emin Maltepe
Jan 15, 2015·Toxicological Research·Aekyong Kim
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Dec 13, 2017·Frontiers in Oncology·Luisa IommariniIvana Kurelac
Mar 14, 2018·Cell Death Discovery·Aastha ChhabraManish Sharma
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Jul 17, 2020·Frontiers in Oncology·Umesh Prasad YadavKapil Mehta
Jan 27, 2021·Trends in Immunology·Long YuanGordon J Freeman

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