PMID: 8952619Sep 1, 1996Paper

Glucose-mediated alteration of cellular function in human periodontal ligament cells

Journal of Dental Research
F NishimuraY Murayama

Abstract

Because diabetic patients are easily led to manifest severe periodontitis, we wanted to determine whether various glucose levels interfere with normal cellular function. Human periodontal ligament (PDL) cells were cultured in glucose-free medium, or in medium containing either 1100 mg/L of glucose (normal-glucose medium) or 4500 mg/L of glucose (high-glucose medium). Cells cultured in glucose-free medium changed their morphology from spindle-shaped to round, and incorporated trypan blue in a time-dependent manner. The incorporation rate was much faster in cells with shorter cell cycles than in those with longer cycles, suggesting the involvement of cell-cycle progression in cell death. However, fragmented DNA, which suggests apoptotic cell death, was not observed in these cells. We reasoned that initial cell rounding and detachment from the culture plate might be due to the conformational changes in cell-surface receptors to fibronectin, a major extracellular matrix for fibroblasts. Western blot analysis revealed that cells cultured in glucose-free medium lost their fibronectin receptor in a time-dependent manner. In addition, fibronectin receptor expression was much higher in cells cultured in high-glucose medium than in cells...Continue Reading

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Citations

Apr 25, 2008·Journal of Dental Research·M QvarnstromJ H Meurman
Oct 17, 1998·Journal of Periodontology·H L CollinJ H Meurman
Nov 2, 2006·Journal of Periodontology·Muthusamy Senthil KumarPraveen Kumar Sehgal
May 23, 2009·Journal of Periodontal Research·R NishiharaK Ito
Aug 20, 2003·Diabetic Medicine : a Journal of the British Diabetic Association·T MarugameS Matsumoto
Mar 27, 2010·Journal of Periodontal Research·T C A PhanM S Goonewardene
Jul 4, 2006·Diabetes Research and Clinical Practice·Hyun Sook KimJo Young Suh
Apr 14, 2016·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L ZhangD Miao
Oct 7, 2000·Journal of Dental Research·F NishimuraY Murayama
Apr 28, 2017·Molecular Medicine Reports·Sujiwan SeubbukRudee Surarit

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