PMID: 8967474May 1, 1996Paper

Glucose metabolism and insulin release in mouse beta HC9 cells, as model for wild-type pancreatic beta-cells

The American Journal of Physiology
Y LiangF M Matschinsky

Abstract

Glucose metabolism and its relationship with glucose-induced insulin release were studied in beta HC9 and beta TC3 cells to identify and characterize key factors controlling the intermediary metabolism of glucose and glucose-induced insulin release. The beta HC9 cell line, derived from pancreatic islets with beta-cell hyperplasia, is characterized by a normal concentration-dependency curve for glucose-stimulated insulin release, whereas the beta TC3 cell line, derived from pancreatic beta-cell tumors, shows a marked leftward shift of this curve. Maximum velocity and the Michaelis-Menten constant of glucose uptake in beta HC9 and beta TC3 cells were similar, even though GLUT-2 expression in these two cell lines differed. In both cell lines, the kinetic characteristics of glucose usage, glucose oxidation, and glucose-induced oxygen consumption were similar to those of glucose phosphorylation, indicating that the kinetics of glucose metabolism from the glucose phosphorylation step in the cytosol to the mitochondrial process of oxidative phosphorylation are determined by the glucose-phosphorylating enzyme, that is, by glucokinase in beta HC9 cells and by hexokinase in beta TC3 cells. Thus beta HC9 cells provide an opportunity for t...Continue Reading

Citations

Nov 6, 2012·Diabetes, Obesity & Metabolism·T J Pullen, G A Rutter
Apr 13, 2018·Journal of Applied Physiology·David F WilsonFranz M Matschinsky
Sep 29, 2011·American Journal of Physiology. Endocrinology and Metabolism·Nicolai M DolibaFranz M Matschinsky
Jun 29, 2017·Physiological Reports·David F WilsonFranz M Matschinsky
Mar 14, 2002·Molecular Aspects of Medicine·G A Rutter

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