Glucose metabolism and metabolic flexibility in blood platelets

Journal of Thrombosis and Haemostasis : JTH
M AibibulaR G Sturmey

Abstract

Essentials The metabolic integration processes required for platelet activation are unclear. The metabolic plasticity of human platelets were investigated. Activated platelets exhibit a glycolytic phenotype while preserving mitochondrial function. Platelets can switch freely between glucose/glycogen and fatty acids to support aggregation. SUMMARY: Background Platelet activation is an energy-dependent process, but the type and integrated use of metabolic fuels required to drive activation remain unclear. Objective To dissect the metabolic fuel and pathway plasticity required for platelet activation. Methods Platelet oxygen consumption rate and extracellular acidification rate were measured as markers of oxidative phosphorylation (OXPHOS) and glycolysis, respectively. Glucose and glycogen were quantified by enzyme-coupled fluorometric assay. Results Blood platelets switched freely between glycolysis and OXPHOS, using either glucose or fatty acids at rest. The transition of platelets from a quiescent to an activated state promoted rapid uptake of exogenous glucose, associated with a shift to a predominantly glycolytic phenotype coupled with a minor rise in mitochondrial oxygen consumption. Consistent with this metabolic plasticity...Continue Reading

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Citations

Feb 23, 2019·Blood Advances·Andaleb Kholmukhamedov, Shawn Jobe
Dec 24, 2019·Journal of Thrombosis and Haemostasis : JTH·Madhumita Chatterjee
Mar 25, 2019·Physiological Research·A T PetrusI Ionită
Nov 19, 2019·Frontiers in Cardiovascular Medicine·Hannah MelchingerJohn Hwa
Aug 13, 2020·International Journal of Molecular Sciences·Elmina Mammadova-BachAttila Braun
Sep 14, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Jessica F Hebert, Leslie Myatt
Jul 13, 2021·Free Radical Biology & Medicine·Renato S GasparAntonio Marcus de A Paes

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