PMID: 3758223Aug 1, 1986Paper

Glucose metabolism by human cataracts in culture

Experimental Eye Research
J K Wolfe, L T Chylack

Abstract

Metabolism in human senile cataracts has been studied using uniformly labeled [14C]glucose. Intracapsularly extracted lenses were cultured in TC-199 media with a glucose concentration of 5.5 mM. Results show that lactate production accounts for 97% of the glucose metabolized. Under these standard incubation conditions there is negligible accumulation of alpha-glycerol phosphate, glucose-6-phosphate, and sorbitol. The rate of lactate production was found to be relatively uniform over a range of cataract severities which were determined from the CCRG classification. The effects of several perturbants in the medium were measured. An ATP concentration of 3 mM was found to inhibit lactate production. Labeled glucose-6-phosphate in the medium was found to produce lactate at a rate approximately one half that of glucose. Elevated glucose concentration resulted in a slight decrease in lactate production and, in some lenses, production of a small amount of sorbitol. Overall, the glycolytic pathway appears to be functioning normally and without regard for cortical and nuclear opacification.

Citations

Jan 1, 1993·The British Journal of Ophthalmology·J J HardingR S Harding
Sep 1, 1988·Experimental Eye Research·B Groth-VasselliP Farnsworth
Mar 1, 1988·Experimental Eye Research·H M Cheng, J B Aguayo
Jul 20, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Jessica A KerrMelissa Wake

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