Glucose metabolism is accelerated by exposure to t-butylhydroperoxide during NADH consumption in human erythrocytes

Blood Cells, Molecules & Diseases
Yuki OgasawaraKazuyuki Ishii

Abstract

Several mechanisms have been proposed to underlie the events that occur during oxidative damage in red blood cells (RBCs) exposed to reactive oxygen species. This work explores what happens when metabolites related to redox regulation in human RBCs are oxidized to form alkoxyl radical and peroxyl radical as a result of exposure to tert-buthylhydroperoxide (BHP). During exposure to BHP, the glutathione level and the ratio of NADPH to total nicotinamide adenine dinucleotide phosphate (NADPH plus NADP(+)) were significantly decreased. Although alteration in the concentration of monosaccharides metabolized in the pentose phosphate pathway (PPP) was not observed, exposing RBCs to BHP caused the formation of methemoglobin (metHb) and a significant decrease in NADH. Moreover, we detected a significant increase in one of the peaks during BHP exposure by using HPLC with dansyl hydrazine as a prelabel reagent. A complete enzymatic conversion procedure was used to identify the peak as pyruvate based on comparison with standards. These results suggest that the rapid recovery in the level of glutathione and the formation of metHb by BHP require NADPH and NADH consumption. Subsequently, glucose metabolism accelerates to reproduce NADPH and N...Continue Reading

References

Oct 15, 1981·Biochemical and Biophysical Research Communications·T YubisuiM Takeshita
May 10, 1995·Archives of Biochemistry and Biophysics·J A ThomasR Honzatko
May 1, 1994·European Journal of Biochemistry·I Schuppe-KoistinenI A Cotgreave
Aug 29, 1997·Biochimica Et Biophysica Acta·C K Lii, C N Hung
Dec 24, 1997·Biological & Pharmaceutical Bulletin·T Matsuoka
Sep 22, 2000·Analytical Biochemistry·Z ZhangR C Stanton
Feb 14, 2004·Redox Report : Communications in Free Radical Research·Joseph M RifkindLuke Babu Ravi
Aug 17, 2006·Antioxidants & Redox Signaling·Argirios E TsantesNikolaos M Sitaras

❮ Previous
Next ❯

Citations

May 26, 2009·Biochemistry·Alexandra IoanoviciuPaul R Ortiz de Montellano
Oct 30, 2012·Clinical Biochemistry·D Polak-JonkiszI Makulska
Aug 19, 2015·Redox Biology·S VoskouM Kleanthous
Jan 29, 2010·The Biochemical Journal·Jolanta Idkowiak-BaldysYusuf A Hannun
Dec 10, 2013·Free Radical Biology & Medicine·Rob van ZwietenDirk Roos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Dorota Polak-JonkiszDanuta Zwolinska
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Daniela BratosinJean Montreuil
Proceedings of the National Academy of Sciences of the United States of America
Anna HanssonNils-Göran Larsson
© 2022 Meta ULC. All rights reserved