Glucose metabolism of human mononuclear cell subpopulations

Pediatric Research
S StorchT Henderson

Abstract

Previous studies have demonstrated metabolic dysfunction in the mononuclear cells of some children with abnormal cell-mediated immunity. Interpretation of these observations has been complicated by the extreme heterogeneity of cell types examined. The glycolytic metabolism of relatively enriched T-cells, non-T mononuclear cells (NTM), non-T lymphocytes (NTL), and monocytes was studied in an attempt to measure the metabolism of subpopulations of mononuclear cells. Lactate production by monocytes was 11 times greater than that of T-cells and 2 1/2 times greater than that of non-T lymphocytes. Exposure to phytohemagglutinin (PHA) stimulated glycolytic metabolism in T-cells but did not stimulate glucose utilization or lactate production in NTM. Even when T-cells were maximally stimulated by PHA, their observed metabolism was still lower than that of NTL. The ATP content of T lymphocytes and NTL was similar and was constant under the conditions of incubation. The initial ATP content of monocytes was higher than that of lymphocytes, and diminished during incubation. Tricarboxylic acid cycle activity did not contribute significantly to ATP synthesis in any of the mononuclear cell subpopulations, under the conditions of incubation used...Continue Reading

Citations

Jan 1, 1983·Journal of Clinical Immunology·H R HillJ I Santos
Apr 11, 1997·Journal of Immunological Methods·G ConstantinE C Butcher
Apr 1, 1984·Experimental Cell Research·D MonosH L Cooper
May 1, 1996·Critical Reviews in Food Science and Nutrition·J Chirife, M D Buera

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