Glucose Oxidase-Instructed Fluorescence Amplification Strategy for Intracellular Glucose Detection

ACS Applied Materials & Interfaces
Shanshan JiangJing Lin

Abstract

The accurate detection of glucose at cellular level remains a big challenge. In this study, a signal amplification strategy mediated by silver nanocube (AgNC), glucose oxidase (GOx), and silver ion fluorescence probe (denoted as AgNC-GOx/Ag+-FP) is proposed for amplified intracellular glucose detection. The AgNC is oxidized into Ag+ by H2O2 generated from GOx-catalyzed glucose oxidation reaction, and Ag+ remarkably enhances the red fluorescence of Ag+-FP. Our results show that AgNC-GOx/Ag+-FP is highly sensitive and specific to glucose and H2O2. Afterward, the feasibility of using AgNC-GOx/Ag+-FP to detect intracellular glucose is verified in five different cell lines. In summary, a sensitive and specific fluorescence amplification strategy has been developed for intracellular glucose detection.

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Citations

Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Chunhua LinYonghai Song
Jun 26, 2021·Analytical and Bioanalytical Chemistry·Chunlei YangXiaoling Zhang
Sep 14, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Qian WangJingcheng Hao

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