Glucose-regulated protein 78 in lipid rafts elevates vascular smooth muscle cell proliferation of spontaneously hypertensive rats by controlling platelet-derived growth factor receptor signaling.

Pflügers Archiv : European journal of physiology
Do-Yoon KimHwan Myung Lee

Abstract

The multifunctional glucose-regulated protein 78 (GRP78) is known to be differentially expressed in the lipid rafts of vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats (SHRs) and normal Wistar-Kyoto (WKY) rats. However, its role in VSMCs from SHRs remains to be elucidated. This work was conducted to investigate the contribution made by GRP78 in VSMCs. GRP78 expression in VSMC lipid rafts decreased in WKY rats with age, but not in SHRs. Transfection with GRP78-siRNA attenuated not only platelet-derived growth factor (PDGF)-BB-induced VSMC proliferation and aortic sprout outgrowth but also the phosphorylation of PDGF receptor (PDGFR)-β, Akt, and extracellular signal-regulated kinase (Erk) 1/2 in VSMCs in response to PDGF-BB. Moreover, GRP78 knockdown also reduced the PDGF-BB-induced dimerization of PDGFR-β and GRP78 in SHR VSMCs. The phosphorylation of GRP78 and PDGFR-β was elevated in VSMCs treated with PDGF-BB and was completely abolished by AG1296 (a PDGFR inhibitor). Moreover, the binding of PDGFR-β to GRP78 and the co-localization of GRP78 to PDGFR-β in VSMCs were stronger in SHRs than in WKY rat controls. This study demonstrates that the PDGF-BB-induced proliferation of SHR VSMCs is mediated by the ...Continue Reading

References

Oct 16, 1999·The Journal of Biological Chemistry·C MineoR G Anderson
Mar 3, 2001·Pathophysiology : the Official Journal of the International Society for Pathophysiology·J J. Dalle LuccaT B. Paiva
Aug 16, 2001·Trends in Biochemical Sciences·A S Lee
Jan 28, 2003·Current Biology : CB·Wia BaronCharles ffrench-Constant
Jun 25, 2004·Journal of Pharmacological Sciences·Seongchun KwonHee Yul Ahn
Feb 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Uma Kant MisraSalvatore Vincent Pizzo
Apr 5, 2005·Methods : a Companion to Methods in Enzymology·Amy S Lee
Jun 21, 2005·Neurobiology of Aging·M Paz GavilánDiego Ruano
Jun 7, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Baohua YangVictor Rizzo
Feb 13, 2007·Current Opinion in Nephrology and Hypertension·Glaucia E CalleraRhian M Touyz
May 15, 2007·Nature·Brett K KaiserThomas Spies
Nov 10, 2007·European Journal of Pharmacology·Hwan Myung LeeBokyung Kim
Mar 30, 2010·Seminars in Cell & Developmental Biology·Pekka MäättänenDavid Y Thomas
Oct 26, 2010·Current Opinion in Cell Biology·Kyle T Pfaffenbach, Amy S Lee
Apr 20, 2011·Expert Review of Proteomics·Simon Staubach, Franz-Georg Hanisch
Nov 2, 2011·Current Cardiology Reviews·Betul Catalgol, Nesrin Kartal Ozer
Oct 30, 2016·Biochimie·Julie ColinThierry Oster
Apr 21, 2017·Frontiers in Neuroscience·Caty Casas
Sep 17, 2017·Oncotarget·Normann SteinerGerold Untergasser
Oct 27, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Changlin WangCheng Zhang
Dec 12, 2017·Protein Science : a Publication of the Protein Society·Valentina L KouznetsovaIgor F Tsigelny
Mar 8, 2018·Pflügers Archiv : European journal of physiology·Kang Pa LeeKyung Jong Won

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
transfection
immunoprecipitation

Software Mentioned

GraphPad
Scion Image
Extract MSN
LSM
GraphPad Prism
MetaMorph

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.