Glucose regulates expression of the nerve growth factor (NGF) receptors TrkA and p75NTR in rat islets and INS-1E beta-cells

Regulatory Peptides
K RaileW Kiess

Abstract

The function and survival of pancreatic beta-cells strongly depend on glucose concentration and on autocrine secretion of peptide growth factors. NGF and its specific receptors TrkA and p75NTR play a pivotal role in islet survival and glucose-dependent insulin secretion. We therefore investigated whether or not glucose concentration influences expression of TrkA and p75NTR in rat islets and in INS-1E beta-cells at the mRNA and protein level (INS-1E). Gene expression of the NGF receptors TrkA and p75NTR but also of the metabolic gene liver-type pyruvate kinase (L-PK) and the neurotrophin receptors TrkB and TrkC was studied by semi-quantitative PCR and by real-time PCR in islets and INS-1E beta-cells. In rat islets, high glucose exposure (25 mmol/l) increased gene expression of TrkA, p75NTR and L-PK. Expression of TrkA, p75NTR and L-PK reflected insulin secretion at the respective glucose concentration. In rat INS-1E insulinoma cells, expression of L-PK and p75NTR was suppressed by low glucose as in the islets, while expression of TrkA was strongly increased by low glucose levels and thus was regulated differently than in islets. Expression of TrkB and TrkC was not regulated by glucose concentration at all. TrkA protein was regul...Continue Reading

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Citations

Sep 22, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Selda Gezginci-Oktayoglu, Sehnaz Bolkent
Nov 3, 2015·Biological & Pharmaceutical Bulletin·Kei MiyagiShogo Tokuyama
Jan 5, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Stefano GovoniEmilio Vanoli
Feb 25, 2015·Diabetes/metabolism Research and Reviews·Daniel LazardKonstantin Bloch
Mar 5, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kurt BoonenLiliane Schoofs
May 8, 2021·Trends in Endocrinology and Metabolism : TEM·Brandon PodymaChristopher D Deppmann
Feb 8, 2011·Neuropeptides·Selda Gezginci-Oktayoglu, Sehnaz Bolkent

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