Glucose reverses 2,4-dinitrophenol induced changes in action potentials and membrane currents of guinea pig ventricular cells via enhanced glycolysis.

Cardiovascular Research
S NakamuraM Arita

Abstract

The precise mechanism involved in the restoration by glucose of action potentials and membrane currents has not been established, so we studied it in single guinea pig ventricular cells under conditions of metabolic inhibition by 2,4-dinitrophenol (DNP). Application of DNP (50 mumol.litre-1) significantly shortened the action potential duration (APD) from 276.3 (SD 47.5) to 43.0(3.9) ms (n = 10). The increase in glucose concentration from 5.5 (control) to 22 mmol.litre-1 significantly restored the APD within 10 min, to 233.2(51.2) ms. In tight seal whole cell clamp studies, we found that DNP increased the steady state outward currents at potentials positive to -60 mV, and that this current was all but suppressed by 22 mmol.litre-1 glucose. The glucose induced recovery in the APD and membrane current was not seen in the presence of 2-deoxyglucose (2 mmol.litre-1), an inhibitor of glycolysis. Insulin (40 mIU.ml-1) reversed the DNP induced shortening of APD and abolished the increase in the outward current in the presence of 11 mmol.litre-1 glucose, though the application of glucose alone at this concentration failed to reverse these changes. Glucose (5.5 to 44 mmol.litre-1) did not directly affect single channel currents of ATP r...Continue Reading

Citations

Jul 9, 2011·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Johann GrundlinghDavid M Wood
Mar 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T HusøyJ Jenssen
Apr 13, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Miyoun HongWilliam A Coetzee
Jul 1, 1991·The Journal of Clinical Investigation·K J Rocha-SinghJ S Karliner
Mar 20, 2016·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Iain P HargreavesSimon J R Heales
Mar 29, 2003·Current Medical Research and Opinion·K BendjelidM Janier

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