Glucose sensor using a phospholipid polymer-based enzyme immobilization method

Analytical and Bioanalytical Chemistry
Hiroyuki KudoKohji Mitsubayashi

Abstract

An electroenzymatic glucose sensor based on a simple enzyme immobilization technique was constructed and tested. The glucose sensor measures glucose concentrations as changes of oxygen concentrations induced by enzymatic reactions. The immobilizing procedure was developed with the purpose of producing wearable biosensors for clinical use. Two types of biocompatible polymers, 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with dodecyl methacrylate (PMD) and MPC copolymerized with 2-ethylhexyl methacrylate, were compared as a sensitive membrane of biosensors. The PMD enzyme membrane had a better response time. Linearity, reproducibility, effect of the concentrations of immobilized enzyme and drifts of sensor characteristics in long-term tests were also investigated. The linear characteristics were confirmed with glucose concentration from 0.01 to 2.00 mmol/l, with a coefficient of determination of 0.9999. The average output current for 1 mmol/l and the standard deviation were 0.992 and 0.0283 muA. Significant changes in the sensor's characteristics were not observed for 2 weeks when it was kept in a refrigerator at 4 degrees C. Because of the simple procedure, the enzyme immobilization method is not only useful for ...Continue Reading

References

Feb 19, 1988·Diabetes Research and Clinical Practice·W L ClarkeL M Simineiro
Sep 1, 1980·The British Journal of Ophthalmology·D K Sen, G S Sarin
Jun 1, 1994·Artificial Organs·H M HeiseF A Gries
Feb 11, 1998·Journal of Biomedical Materials Research·K IshiharaN Nakabayashi
Aug 5, 2000·Biochemical and Biophysical Research Communications·S D'AuriaJ R Lakowicz
Oct 16, 2003·Biosensors & Bioelectronics·Kohji MitsubayashiTatsuro Endo
Mar 3, 2005·Biosensors & Bioelectronics·John C PickupNabihah Sachedina
Apr 28, 2005·Biosensors & Bioelectronics·George S Wilson, Raeann Gifford
Jun 17, 2006·Biosensors & Bioelectronics·Hiroyuki KudoKohji Mitsubayashi
May 24, 2007·Biomedical Microdevices·Shigehito IguchiKohji Mitsubayashi
Jun 15, 2007·Diabetes Technology & Therapeutics·Timothy S BaileySatish K Garg
Feb 1, 1988·Talanta·D Meadows, J S Schultz
Sep 1, 2003·Journal of Fluorescence·Ramachandram BaduguChris D Geddes
Dec 1, 1997·Sensors and Actuators. B, Chemical·Leah TolosaJoseph R Lakowicz

❮ Previous
Next ❯

Citations

Apr 9, 2011·Biomedical Microdevices·MingXing ChuKohji Mitsubayashi
Aug 23, 2020·Sensors·William S Skinner, Keat Ghee Ong
May 13, 2019··Kohji Mitsubayashi, Kohji Mitsubayashi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.