Glucose-stimulated insulin secretion correlates with beta-cell lipolysis

Nutrition, Metabolism, and Cardiovascular Diseases : NMCD
Maria Sörhede WinzellBo Ahrén

Abstract

Lipids are needed for optimal glucose-stimulated insulin secretion (GSIS), and long-chain acyl-CoA (LC-CoA) has been suggested as one candidate molecule active as a lipidic coupling factor. LC-CoAs may be available to the beta-cell via uptake of circulating free fatty acids or from hydrolysis of intracellularly stored triglycerides. Inhibition of lipolysis in rat islets using a non-specific lipase inhibitor (orlistat) resulted in blunted GSIS. The aim of this study was to investigate the relationship between GSIS and lipolysis in clonal beta-cells and in mouse islets. INS-1 cells, cultured overnight at 3.3 mM or 11.1 mM glucose, or freshly isolated islets were incubated with 3.3 mM or 16.7 mM glucose for 1 h. Medium samples were collected and analyzed for insulin and glycerol. Triglycerides were measured in both INS-1 cells and islets. There was a dose-dependent glucose-stimulated lipolysis in INS-1 cells, which strongly correlated with insulin secretion (r=0.85, P<0.0001). The same phenomenon was observed in mouse islets (r=0.9, P=0.013). Low levels of triglycerides, which were observed in INS-1 cells pre-cultured at 3.3 mM glucose, were associated with reduced GSIS. This study suggests that lipids obtained from lipolysis of i...Continue Reading

References

Feb 15, 1989·Analytical Biochemistry·J HellmérA Lundin
Jan 1, 1994·Journal of Cellular Biochemistry·F M AshcroftP Rorsman
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J OsugaN Yamada
Mar 18, 2000·The Journal of Nutrition·B E CorkeyM Prentki
Apr 24, 2001·Obesity Research·S P WangG A Mitchell
Nov 22, 2001·The Journal of Biological Chemistry·Guenter HaemmerleRudolf Zechner
Sep 19, 2003·Diabetologia·G C Yaney, B E Corkey
Jun 29, 2004·The Journal of Biological Chemistry·Krishnakant G SoniGrant A Mitchell
Nov 20, 2004·Science·Robert ZimmermannRudolf Zechner

❮ Previous
Next ❯

Citations

Apr 25, 2009·The Journal of Biological Chemistry·Marie-Line PeyotMarc Prentki
Sep 2, 2016·Physiological Reviews·David G Nicholls
Jun 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Petr JežekLydie Plecitá-Hlavatá
Sep 17, 2008·Biochemical Society Transactions·Malin Fex, Hindrik Mulder
Jul 26, 2018·Frontiers in Physiology·Giuseppe CalamitaChristine Delporte
Feb 13, 2021·Antioxidants·Petr JežekLydie Plecitá-Hlavatá
Mar 11, 2021·Biochimie·Giuseppe Calamita, Christine Delporte
Jun 29, 2021·Antioxidants & Redox Signaling·Petr JežekLydie Plecitá-Hlavatá

❮ Previous
Next ❯

Related Concepts

Related Feeds