Glucose transport system in UMR-106-01 osteoblastic osteosarcoma cells: regulation by insulin

Calcified Tissue International
E A ItuarteT J Hahn

Abstract

Insulin is a potent stimulator of collagen synthesis and other osteoblastic cell functions. In various insulin-sensitive tissues, stimulation of glucose transport and glycolytic metabolism are hallmarks of insulin action and may play a role in insulin regulation of cellular function. However, the effects of insulin on glucose metabolism in osteoblast-like cells have not been defined. We therefore characterized 2-deoxy-D-glucose (2-DG) transport in UMR-106-01 rat osteoblastic osteosarcoma cells and examined its regulation by insulin. 2-DG (0.1 mM) uptake was shown to be linear with time over 45 minutes, temperature-sensitive, and inhibited by phloridzin. Competitive inhibition studies against other hexoses demonstrated a transport system stereospecificity for 2-DG similar to that previously demonstrated in fat and muscle cells. Kinetic analysis of 15 minute 2-DG uptake at 25 degrees C demonstrated a saturable transport mechanism with a Km (1.9 mM) similar to that observed for 2-DG transport in other tissues. Insulin stimulated 2-DG transport in a dose-related manner, with significant stimulation observed at 0.5 nM and maximal effect observed at 50 nM insulin. The stimulatory effect of insulin was reversibly inhibited by cytochal...Continue Reading

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Citations

Nov 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A J FelsenfeldT J Hahn
Mar 7, 2007·Calcified Tissue International·H XieE-Y Liao
Oct 11, 2005·American Journal of Physiology. Endocrinology and Metabolism·Kathryn M ThrailkillJohn L Fowlkes
Sep 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D M ThomasJ D Best
Apr 1, 2014·Endocrine Research·Sakarat N PramojaneeSiriporn C Chattipakorn
May 15, 2010·Clinical Oral Implants Research·M Retzepi, N Donos
Jun 1, 2011·Clinical Endocrinology·Kong Wah Ng
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Jun 26, 2014·Journal of Diabetes Research·Kathryn ThrailkillJeffry S Nyman
Jul 5, 2011·Bone·Keertik Fulzele, Thomas L Clemens
Nov 25, 2014·Calcified Tissue International·Tara C Brennan-Speranza, Arthur D Conigrave
Apr 16, 2015·International Journal of Endocrinology·Maria Felicia FaienzaGiacomina Brunetti
Feb 7, 2015·The Journal of Endocrinology·K J OldknowC Farquharson
Sep 28, 2005·Diabetes·Kathryn M ThrailkillCharles K Lumpkin
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