Glutamate activates GFAP gene promoter from cultured astrocytes through TGF-beta1 pathways

Journal of Neurochemistry
Luciana Ferreira RomãoFlávia Carvalho Alcantara Gomes

Abstract

Glial cells are currently viewed as active partners of neurons in synapse formation. The close proximity of astrocytes to the synaptic cleft suggests that these cells might be potential targets for neuronal-released molecules although this issue has been less addressed. Here, we evaluated the role of the excitatory neurotransmitter, glutamate, in astrocyte differentiation. We recently demonstrated that cortical neurons activate the gene promoter of the astrocyte maturation marker, GFAP (glial fibrillary acidic protein) of cerebral cortex astrocytes by inducing TGF-beta1 (transforming growth factor beta 1) secretion in vitro. To access the effect of glutamate on GFAP gene, we used transgenic mice bearing the beta-Galactosidase (beta-Gal) reporter gene under the regulation of the GFAP gene promoter. We report that 100 muM glutamate activates the GFAP gene promoter of astrocytes from cerebral cortex revealed by a significant increase in the number of beta-Gal positive astrocytes. Neutralizing antibodies against TGF-beta completely prevented glutamate and neuronal-induction of GFAP gene, thus indicating that this event is mediated by TGF-beta1. Further, induction of GFAP gene in response to glutamate was followed by nuclear translo...Continue Reading

References

Mar 1, 1995·Journal of Neuroscience Research·J Garcia-AbreuL A Cavalcante
Jan 1, 1995·Neuropharmacology·J P Pin, R Duvoisin
Mar 1, 1997·Progress in Neurobiology·J T Porter, K D McCarthy
Sep 12, 1997·Science·F W Pfrieger, B A Barres
Jul 17, 1998·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·F R LimaV Moura Neto
Oct 3, 1998·The European Journal of Neuroscience·U StrasserD W Choi
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·D A Lim, A Alvarez-Buylla
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F C GomesV Moura Neto
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·J Massagué
Nov 10, 2001·Science·D H MauchF W Pfrieger
Sep 7, 2002·Science·Mary E Hatten
Oct 10, 2002·The European Journal of Neuroscience·Karen SchlüterJürgen Engele
Dec 11, 2002·The European Journal of Neuroscience·Tânia Cristina Leite de Sampaio e SpohrFlávia Carvalho Alcantara Gomes
Feb 4, 2003·Nature Reviews. Neuroscience·Mary PackardVivian Budnik
Mar 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ralf S SchmidEva S Anton
Apr 30, 2003·Journal of Neurobiology·Leonardo C deAzevedoRoberto Lent
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Apr 14, 2004·The European Journal of Neuroscience·Vivian de Oliveira SousaFlávia Carvalho Alcantara Gomes
Dec 14, 2004·Neuroscience·R LujánG López-Bendito
Feb 15, 2005·Cell·Karen S ChristophersonBen A Barres
Jun 7, 2005·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Flávia Carvalho Alcantara GomesLuciana Romão
Sep 8, 2005·Current Opinion in Neurobiology·Nicola J Allen, Ben A Barres
Oct 28, 2005·Nature·Dieter-Chichung LieFred H Gage
Nov 8, 2005·Cytokine & Growth Factor Reviews·Denis Vivien, Carine Ali
Feb 24, 2006·Nature Reviews. Neuroscience·Gerald SeifertChristian Steinhäuser
May 9, 2006·FEBS Letters·Joan Massagué, Roger R Gomis
Sep 5, 2006·In Vitro Cellular & Developmental Biology. Animal·Vivian De Oliveira SousaFlávia Carvalho Alcantara Gomes
Dec 16, 2006·Current Alzheimer Research·Ina Tesseur, Tony Wyss-Coray

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Citations

Mar 28, 2013·Neurotoxicity Research·Arik EisenkraftArseny Finkelstein
Oct 15, 2009·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Peter T OharaLuc Jasmin
Aug 8, 2013·PloS One·Kim M LeeAndrew G MacLean
May 23, 2012·Neurochemical Research·Joice StipurskyFlávia Carvalho Alcantara Gomes
Jul 1, 2012·Open Journal of Apoptosis·Madhusudhanan NarasimhanLenin Mahimainathan
Dec 9, 2014·Frontiers in Cellular Neuroscience·Joice StipurskyFlávia Carvalho Alcantara Gomes
May 21, 2016·Frontiers in Cellular Neuroscience·Ana P B AraujoFlávia C A Gomes
Jun 25, 2016·BMC Biophysics·Yareni A AyalaH Moysés Nussenzveig
Oct 21, 2016·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Egle Cekanaviciute, Marion S Buckwalter
May 20, 2017·Molecular Neurobiology·Michele SiqueiraJoice Stipursky
Jan 3, 2015·Biochimica Et Biophysica Acta·Joana Balça-SilvaMaria Celeste Lopes
Dec 23, 2015·Inflammatory Bowel Diseases·Fernando Ochoa-CortesFievos L Christofi
Feb 24, 2015·Glia·Juliana de Mattos Coelho-AguiarVivaldo Moura-Neto
Sep 12, 2009·Journal of Neuroscience Research·Tânia Cristina Leite de Sampaio e SpohrFlávia Carvalho Alcantara Gomes
Feb 24, 2016·Bulletin of Experimental Biology and Medicine·D N VoronkovL I Sheloukhova
May 17, 2012·Journal of Neuroscience Research·Tânia Cristina Leite de Sampaio E SpohrStevens Kastrup Rehen
Jun 2, 2012·Developmental Neuroscience·Joice StipurskyFlávia Carvalho Alcantara Gomes
Nov 1, 2018·Molecular Neurobiology·Luan Pereira DinizFlávia Carvalho Alcantara Gomes
Jun 21, 2019·The Cerebellum·Ana Paula Bergamo AraujoFlávia Carvalho Alcantara Gomes
Jan 5, 2017·Molecular Neurobiology·Andrea Schmidt BuosiFlávia Carvalho Alcantara Gomes
May 16, 2019·Experimental and Therapeutic Medicine·Weihua YinMaoying Fu
Jan 30, 2021·Frontiers in Pharmacology·Maurício Dos Santos PereiraElaine Del Bel
Jan 23, 2021·ACS Chemical Neuroscience·Ha-Rim LeeHyun-Jung Kim
May 17, 2011·Life Sciences·Joice StipurskyFlávia Carvalho Alcantara Gomes
Jul 3, 2021·Cells·Anastasia A BorodinovaOlga L Vlasova

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