Glutamate and aspartate immunoreactivity in corticospinal neurons of rats.

The Journal of Comparative Neurology
R Giuffrida, A Rustioni

Abstract

A combination of retrograde tracers and immunostaining was employed to test whether corticospinal tract (CST) neurons in rats may use amino acid excitatory neurotransmitters. CST neurons were identified following injections of either Diamidino Yellow (DY) or colloidal gold-labeled enzymatically inactive horseradish peroxidase conjugated to wheat germ agglutinin (WGAapoHRP-Au) in the spinal cord. As retrograde tracers, the two substances seemed to be equally effective, but WGAapoHRP-Au was better suited than DY as a tracer to use in combination with immunocytochemistry. Sections through the primary sensorimotor cortex, which contained the bulk of identified CST neurons, and the secondary somatosensory cortex were processed with antisera raised in rabbits against glutamate (Glu) or aspartate (Asp) conjugated by glutaraldehyde to hemocyanin. In rats with DY injections, about 60-75% of the CST neurons were Glu-immunopositive, with higher ratios in SI and MI than in SII. Similar results were obtained in all areas examined from the rats with injections of WGAapoHRP-Au. Only sections from rats with injections of WGAapoHRP-Au were processed for Asp immunostaining. In this material, between 65 and 75% of the CST neurons were Asp-immunop...Continue Reading

References

Mar 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J E MadlA J Beitz
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S De Biasi, A Rustioni
Nov 8, 1988·The Journal of Comparative Neurology·G Battaglia, A Rustioni
Apr 19, 1985·Neuroscience Letters·R J WeinbergA Rustioni
Jan 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J R HeplerP Petrusz
Jan 1, 1987·Experimental Brain Research·M W Miller
Jan 1, 1984·Journal of Neurochemistry·F Fonnum
Nov 20, 1982·The Journal of Comparative Neurology·J P Donoghue, S P Wise

❮ Previous
Next ❯

Citations

Dec 1, 1992·Neurochemical Research·G VoukelatouE D Kouvelas
Jul 19, 1991·Brain Research·G A Kevetter, R D Hoffman
Oct 20, 1994·Behavioural Brain Research·F Conti, T Manzoni
Sep 1, 1995·Progress in Neuro-psychopharmacology & Biological Psychiatry·L DíazG Bustos
Jan 2, 1991·Neuroscience Letters·A DinopoulosJ G Parnavelas
Feb 25, 1991·Neuroscience Letters·G A Kevetter, A R Coffey
Jan 1, 1991·Neuroscience·D J TraceyA Rustioni
Jun 1, 2005·Progress in Neurobiology·F BlockM Loos
Jan 7, 1998·Trends in Neurosciences·J R Wolpaw
Apr 30, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R GiuffridaS Sapienza
Jan 1, 1993·Journal of Neurotrauma·P S Fishman, A Mattu
Apr 18, 2001·Clinical Neuropharmacology·J T Greenamyre
Jan 11, 1991·Neurochemical Research·R K SimpsonJ C Goodman
May 10, 2012·Experimental Neurology·Sandra KapitzaMartin E Schwab
Jul 1, 2008·IEEE Transactions on Neural Systems and Rehabilitation Engineering : a Publication of the IEEE Engineering in Medicine and Biology Society·Fivos PanetsosVanessa Bonacasa
Jul 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M MarsalaT L Yaksh
Aug 22, 1992·The Journal of Comparative Neurology·K N WestlundW D Willis
Sep 1, 1993·The Journal of Comparative Neurology·G F Tseng, D A Prince
Apr 13, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Baohan PanLawrence P Schramm
Oct 23, 1995·The Journal of Comparative Neurology·H A Lekan, S M Carlton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Experimental Brain Research
J G ValtschanoffA Rustioni
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J R HeplerP Petrusz
Electroencephalography and Clinical Neurophysiology
P J Delwaide, C A Penders
© 2022 Meta ULC. All rights reserved