Oct 1, 1989

Glutamate and aspartate immunoreactivity in corticospinal neurons of rats

The Journal of Comparative Neurology
R Giuffrida, A Rustioni

Abstract

A combination of retrograde tracers and immunostaining was employed to test whether corticospinal tract (CST) neurons in rats may use amino acid excitatory neurotransmitters. CST neurons were identified following injections of either Diamidino Yellow (DY) or colloidal gold-labeled enzymatically inactive horseradish peroxidase conjugated to wheat germ agglutinin (WGAapoHRP-Au) in the spinal cord. As retrograde tracers, the two substances seemed to be equally effective, but WGAapoHRP-Au was better suited than DY as a tracer to use in combination with immunocytochemistry. Sections through the primary sensorimotor cortex, which contained the bulk of identified CST neurons, and the secondary somatosensory cortex were processed with antisera raised in rabbits against glutamate (Glu) or aspartate (Asp) conjugated by glutaraldehyde to hemocyanin. In rats with DY injections, about 60-75% of the CST neurons were Glu-immunopositive, with higher ratios in SI and MI than in SII. Similar results were obtained in all areas examined from the rats with injections of WGAapoHRP-Au. Only sections from rats with injections of WGAapoHRP-Au were processed for Asp immunostaining. In this material, between 65 and 75% of the CST neurons were Asp-immunop...Continue Reading

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Mentioned in this Paper

Alpha-Peroxidase
Immunoreactivity
Aspartic Acid, Magnesium-Potassium (2:1:2) Salt
Aspartate
Wheat Germ Agglutinin Isolectin 2
Malignant Neoplasm of Spinal Cord
August Rats
Neurons
Secondary Somatosensory Cortex
Immune Sera

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