Glutamate and aspartate measurements in individual planaria by rapid capillary electrophoresis

Journal of Pharmacological and Toxicological Methods
Chandni A VyasJonathan G Shackman

Abstract

Planaria present a unique model organism for studying primitive central nervous systems. The major mammalian excitatory neurotransmitters, glutamate and aspartate, have previously been measured in planaria via high pressure liquid chromatography (HPLC). A faster extraction and analysis procedure using capillary electrophoresis (CE) was developed which confirms the presence of these amino acids in single planaria homogenates. Following homogenization and centrifugation of individual planaria in hydrochloric acid/acetonitrile, glutamate and aspartate were derivatized with naphthalene-2, 3-dicarboxaldehyde (NDA). The labeled amino acids were measured using capillary electrophoresis with laser-induced fluorescence (CE-LIF). CE-LIF electropherograms were generated in less than 1 min. The mean ± S.D. amounts of glutamate and aspartate were 1200 ± 500 and 1900 ± 700 pmol/mg-planarian (n=22), respectively. Spiked average recoveries of glutamate and aspartate were 96% and 91%, respectively. The high-throughput method provides the ability to quantitate changes in excitatory neurotransmitters under developmental or stimulatory conditions. The capability to monitor multiple neurotransmitter levels offers the opportunity to correlate behavi...Continue Reading

References

Nov 1, 1995·Electrophoresis·Z K Shihabi, M E Hinsdale
Mar 16, 2000·Neuroreport·M T Itoh, J Igarashi
Aug 16, 2000·Neuroreport·T Kusayama, S Watanabe
Jan 16, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·F R ButtarelliG Palladini
Jul 2, 2003·Journal of Pharmacological Sciences·Yoshihisa KitamuraKenji Watanabe
Jul 3, 2004·Journal of Chromatography. a·Jonathan G ShackmanRobert T Kennedy
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Peter W Reddien, Alejandro Sánchez Alvarado
Dec 29, 2004·Brain Research·Robert B Raffa, Andrea F Martley
Dec 8, 2005·Journal of Pharmacological and Toxicological Methods·Scott M RawlsRobert B Raffa
Jun 10, 2006·Trends in Pharmacological Sciences·Ulrik GetherArne Schousboe
Aug 18, 2006·Brain Research·Robert B RaffaRonald J Tallarida
Jun 26, 2007·Trends in Neurosciences·Colin J Akerman, Hollis T Cline
Jan 29, 2008·Pharmacology, Biochemistry, and Behavior·Oné R PagánLilly K Afshari
Feb 26, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Francesca R ButtarelliFrancesco E Pontieri
May 31, 2008·Neuroscience Letters·Steve SacavageScott M Rawls
Oct 15, 2009·Journal of Neural Transmission·Marina Sorrentino Hernandes, Lanfranco R P Troncone
Jul 19, 2008·Annual Review of Analytical Chemistry·Kristin N Schultz, Robert T Kennedy
Jan 20, 2010·ACS Chemical Neuroscience·David E Featherstone

❮ Previous
Next ❯

Citations

Aug 10, 2013·Analytical and Bioanalytical Chemistry·Xiangtang LiYi-Ming Liu
Jan 21, 2014·Neuropharmacology·Christopher S TallaridaScott M Rawls
Jun 28, 2011·Pharmacology, Biochemistry, and Behavior·Latha Ramakrishnan, Cassie Desaer
Apr 17, 2015·PloS One·Dimitrios MoustakasEvelyn B Voura
Jan 4, 2012·Electrophoresis·Véréna PoinsotFrançois Couderc
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.