PMID: 4375961May 1, 1974Paper

Glutamate and aspartate transport in rat brain mitochondria

The Biochemical Journal
M D Brand, J B Chappell


1. Rat brain mitochondria did not swell in iso-osmotic solutions of ammonium or potassium (plus valinomycin) glutamate or aspartate, with or without addition of uncouplers. 2. Glutamate was able to reduce intramitochondrial NAD(P)(+); aspartate was able to cause partial re-oxidation. 3. These effects were inhibited by threo-hydroxy-aspartate in whole but not in lysed mitochondria. 4. The existence of a ;malate-aspartate shuttle' for the oxidation of extramitochondrial NADH was demonstrated. This shuttle requires the net exchange of glutamate for aspartate across the mitochondrial membrane. 5. Extramitochondrial glutamate did not inhibit intramitochondrial glutaminase under conditions in which the inhibition in lysed mitochondria was virtually complete. 6. The glutaminase activity of these mitochondria was not energy-dependent. 7. We conclude that these mitochondria do not possess a glutamate-hydroxyl antiporter similar to that of liver mitochondria nor a glutamate-glutamine antiporter similar to that of pig kidney mitochondria, but that they do possess a glutamate-aspartate antiporter.


May 1, 1979·Journal of Neurochemistry·J OrlickýH Hager
Nov 1, 1977·Journal of Neurochemistry·A Minn, J Gayet
Jul 1, 1982·Journal of Neurochemistry·M RuscákH Hager
May 1, 1988·Journal of Neurochemistry·A J Cheeseman, J B Clark
Jan 7, 2015·Journal of Biomolecular Structure & Dynamics·Kalyani KorlaChanchal K Mitra
Feb 26, 2011·Cardiovascular Research·Torsten Toftegaard NielsenHans Erik Bøtker
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mark A MintunMarcus E Raichle
Apr 1, 1976·Archives of Biochemistry and Biophysics·M E TischlerK F La Noue
Feb 1, 1987·Neurochemical Pathology·A J Cooper, J C Lai
Jun 29, 1984·Biochemical and Biophysical Research Communications·S PassarellaE Quagliariello
Sep 1, 1978·Journal of Neurochemistry·S G Dennis, J B Clark
Mar 1, 1987·Neurochemical Research·S PassarellaE Quagliariello
Aug 14, 2020·Physiology·Edmund R S KunjiChancievan Thangaratnarajah
Feb 16, 2013·Neurochemical Research·Jason R TrebergDirk Weihrauch
Nov 12, 1996·Biochimica Et Biophysica Acta·M ErecińskaI A Silver
Oct 1, 1984·Journal of the Neurological Sciences·C J Carter

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.