Glutamate and histidine improve both solvent yields and the acid tolerance response of Clostridium beijerinckii NCP 260

Journal of Applied Microbiology
B W P Reeve, Sharon J Reid

Abstract

This study aims to examine the effect of amino acid supplementation on solvent production by Clostridium beijerinckii during the acetone-butanol fermentation and to determine whether amino acids are involved in the acid tolerance response (ATR), which results in increased solvents. Fermentation studies with Cl. beijerinckii NCP 260 in limited-nitrogen media supplemented with glutamate, glutamine, lysine, proline, histidine or asparagine revealed that only glutamate, glutamine or histidine increased butanol titres comparable to control media. Acid survival tests at pH 5 showed that glutamate and histidine were effective in protecting Cl. beijerinckii cells against acid shock, and may be involved in the ATR. Using quantitative PCR, the transcription of the glutamine synthetase, nitrogen regulator and glutamate synthase operon (glnA-nitR-gltAB) was monitored during acid shock conditions, and expression of both the nitR and gltA genes was shown to be increased twofold. Glutamate and histidine specifically enhance the ATR in Cl. beijerinckii NCP 260, and the genes encoding glutamate synthase and the NitR regulator are both upregulated, predicted to lead to increased endogenous glutamate pools during acidogenesis. This may enhance th...Continue Reading

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Citations

Nov 28, 2019·Applied Microbiology and Biotechnology·Ningzi Guan, Long Liu
Feb 11, 2021·Antonie van Leeuwenhoek·Carmen HogendoornHuub J M Op den Camp
Apr 27, 2021·Applied Microbiology and Biotechnology·Mamou DialloAna M López-Contreras

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